摘要
目的研究螺旋藻对阿霉素肾硬化大鼠肾脏的保护作用及相关机制。方法将32只SD大鼠随机分为4组:假手术组(S组),阿霉素肾硬化对照组(M组),螺旋藻组(SP组),贝那普利治疗组(B组)。8周后观察各组大鼠24 h尿蛋白定量、血尿素氮(BUN)、肌酐(Scr)及肾脏病理改变,免疫组化观察肾脏组织纤连蛋白(FN)、IV型胶原(Col IV)、转化生长因子β1(TGF-β1)的表达。RT-PCR测定肾皮质TGF-β1mRNA的表达。结果螺旋藻能减少尿蛋白、降低BUN和Scr,减轻基质增生和肾小球硬化,且能减少FN、Col IV、TGF-β1的表达。下调肾皮质TGF-β1mRNA的表达。结论螺旋藻能减轻阿霉素肾硬化大鼠肾脏损害,对肾脏有保护作用。
Objective： To investigate the effects and mechanism of spirlina on adriamycin-induced glomerulosclerosis in rats. Methods： Thirty-two Sprague Dawley rats were randomly divided into sham operation,glomerulosclerosis model,spirlina treatment and benazepril groups. The 24-hour urinary protein excretion,Blood urea nitrogen（ BUN） and creatinine were measured after 8 weeks. Renal pathology and immunohistochemistry were assayed to measure the expression of fibronectin（ FN）,collagenIV（ Col IV） and transformer growth factorβ1（ TGF-β1）. RT-PCR was used to examine the TGF-β1mRNA. Results： The proteinuria,BUN and serum creatinine declined significantly（ P 0. 01） and lowed the expressions of FN,Col IV,TGF-β1and TGF-β1mRNA（ P 0. 01）. Conclusion： Spirlina plays an important role in the prevention and treatment for glomerulosclerosis.
出处
《泰山医学院学报》
CAS
2014年第3期168-170,共3页
Journal of Taishan Medical College
基金
泰安市科技局科研计划项目(NO.2008)
