摘要
目的 研究高浓度氧暴露致新生大鼠急性肺损伤时血管内皮生长因子（VEGF）和血管生成素（Ang）的表达及其对肺发育的影响,为临床防治支气管肺发育不良提供理论依据.方法 生后2～3日龄的新生SD大鼠共48只随机分为正常组、高氧组各24只,分别置于空气及常压高浓度氧气（体积分数95％以上）喂养,采用荧光定量PCR和Western Blot方法检测实验第1天、第3天、第7天时两组鼠肺组织VEGF和Ang的mRNA和蛋白水平的表达,苏木精-伊红染色对照动态观察新生大鼠肺组织形态结构变化.结果 高氧组肺组织VEGF和Ang mRNA表达水平第1天（0.86±0.3）、（0.63±0.3）,第3天（0.54±0.26）、（0.91±0.3）,第7天（0.32±0.20）、（0.42±0.1）,均明显低于正常组第1天（1.00±0.28）、（1.00 ±0.24）,第3天（0.92±0.34）、（1.32±0.18）,第7天（0.61±0.12）、（0.72±0.32）,差异均有统计学意义（P＜0.05）;高氧组肺组织VEG和Ang蛋白表达水平第1天（0.78±0.3）、（0.65±0.16）,第3天（0.60±0.08）、（0.65±0.16）,第7天（0.34±0.12）、（0.32±0.14）,均明显低于正常组第1天（1.00±0.16）、（1.00±0.37）,第3天（0.85±0.27）、（1.40±0.25）,第7天（0.56±0.18）、（0.65±0.36）,差异均有统计学意义（P＜0.05）.与正常组相比,高氧组肺组织病理形态分析,肺组织显著发育不良,肺泡结构简单化,肺泡数目减少和肺微血管发育受阻.结论 VEGF和Ang对肺发育起着重要作用,且VEGF和Ang在肺发育的过程中可能起着相互协同和制约的作用.
Objective To explore the effect and levels of VEGF and Ang for lung development from lung injure of hyperoxia in model rats of ALI.Methods Forty-eight Sprague-Dawley neonatal rats （2 to 3-days-old） were simply randomized and raised with hyperoxia（≥95％） or air for 1,3 and 7 days respectively and the VEGF and Ang mRNA and protein expression in lung tissues were analysed with RT-PCR and Western blot.Histologic analysis of Iung were taken according to the HE staining.Results Hyperoxia lung tissue VEGF and Ang mRNA expression levels on 1 day （0.86 ± 0.3）,（0.63 ± 0.3） ; 3 days （0.54 ± 0.26）,（0.91 ±0.3） ; 7 days （0.32 ± 0.20）,（0.42 ±0.1） were significantly lower than the control group on 1 day （1.00 ±0.28）,（1.00 ±0.24）;3 days （0.92 ±0.34）,（1.32 ±0.18）,7 days （0.61 ±0.12）,（0.72 ±0.32）,there were significant differences （P ＜ 0.05） ; hyperoxia group on 1 day （0.78 ±0.3）,（0.65 ±0.16） ; 3 days （0.60 ±0.08）,（0.65 ±0.16） ; 7 days （0.34 ±0.10）,（0.32 ± 0.14） in neonatal rat lung tissue protein expression of VEGF and Ang were significantly lower than the control group1 day （1.00±0.16）,（1.00±0.37）; 3 days （0.85±0.27）,（1.40±0.25）; 7 days （0.56 ± 0.18）,（0.65 ± 0.36） ; there were significant differences （P ＜ 0.05）.The lung tissue of hyperoxia groups in neonatal rats became worse than the tissue in control,and showed simply alveoli structure,thiner septa and walls of alveoli and decreased alveoli.Conclusion VEGF and Ang may take an important part in lung development from lung injure of hyperoxia,and they may cooperate or restrict with each other.
出处
《中华损伤与修复杂志（电子版）》
CAS
2014年第1期37-41,共5页
Chinese Journal of Injury Repair and Wound Healing(Electronic Edition)
