摘要
目的:筛选蓝刺头多糖的除蛋白工艺。方法:以蛋白清除率和多糖保留率为指标,比较了三氯乙酸法(TCA法)、Sevage法、木瓜蛋白酶法的除蛋白效果,并利用响应面法选取反应温度、时间、pH为因素优化了木瓜蛋白酶法除蛋白的最佳工艺。结果:TCA法和Sevage法的最佳蛋白清除率分别为45.13%和47.47%,其多糖保留率分别为61.05%、67.65%,优化的木瓜蛋白酶法除蛋白最佳工艺为酶解温度64℃,时间2.6h,pH6.0,在此条件下的蛋白清除率为56.57%,多糖保留率为98.12%。结论:综合考察各指标,说明木瓜蛋白酶法除蛋白方法效果较好,为蓝刺头多糖制备工艺的进一步研究提供了参考。
Objective：To select deproteinization-technology of Echinops latifolius Tausch polysaccharides. Methods：These different ways to removing protein from polysaccharides in Echinops latifolius Tausch contain trichloroacetic acid（TCA） method,Sevage method and enzymatic method,and that were compared with the clearance rate of protein and the polysaccharide retention as indexes,and the enzymatic method was optimized by response surface analysis. Results：The protein removal rats of TCA and Sevage methods were 45.13% and 47.47% ,and the polysaccharide retention rats were 61.05% ,67.65% ,respectively. and the optimum technologic conditions of deproteinization were the pH6.0,64℃ for 2.6h. under these conditions the protein removal and polysaccharide retention rats were 56.57% and 98.16% ,Conclusion ：The enzyme method was the best deproteinization method of the polysaccharide extracts from Echinops latifolius Tausch.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第10期287-291,共5页
Science and Technology of Food Industry
关键词
蓝刺头多糖
除蛋白
木瓜蛋白酶法
响应面分析法
Echinops latifolius Tausch polysaccharides
deproteinize
enzymatic extraction
response surfaceanalysis
