摘要
从广西8个甘蔗主产区分离得到10个DSE菌株。这些菌株的核糖体DNA的28S基因D1/D2区序列与Genbank中最相似序列构建的系统发育树结果表明:10个菌株在系统树上聚为3个大分支。其中4个菌株以68%的支持率与枝孢属Cladosporium、茎点霉属Phoma、Lophiostoma sp.和Emericella sp.聚为一大类;5个菌株在84%的支持率上与Conlarium sp.、Cercophora sp.和Schizothecium sp.聚为一大类;1个菌株与Phialocephala fortinii聚为一类,支持率为100%。10个菌株均能与甘蔗共生而无致病性,其中7个菌株接种甘蔗组培苗平均干重高于对照,选取干重最高的2个菌株接种甘蔗盆栽苗,接种处理的平均株高、茎宽、鲜重和干重均高于对照。
Ten DSE strains were isolated from eight main sugarcane production areas of Gaungxi.The neighbor-joining phylogenetic tree was produced based on sequences of the 28S rDNA gene D1/D2 region of the 10 strains and the related sequences from the GenBank.The results showed that 10 isolates could be divided into three groups in the phylogenetic tree.Four isolates were clustered with Cladospodum sp.,Phoma sp.,Lophiostoma sp.and Emericella sp.at the support rate of 68％.Five isolates were grouped with Conlarium sp.,Cercophora sp.and Schizothecium sp.at the support rate of 84％ and one isolate was clustered with Phialocephalafonini with the support rate of 100％.All of the isolates showed no pathogericity to sugarcane seedling in vitro.The average biomass of seven isolates was higher than that of the control,and two highest isolates were selected to inoculate with pot seedlings.The average height,stalk width,fresh weight and biomass of the inoculated pot seedlings were higher than those of the control.
出处
《西南农业学报》
CSCD
北大核心
2014年第2期635-640,共6页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金项目(31060007)
农业部"引进国际先进农业科学技术"项目(2012-Z51)
广西自然科学基金项目(2012GXNSFAA053049)
广西农业科学院基本科研业务专项[201040(基)]
