摘要
传统的形态学分类很难鉴定昆虫的蛹和幼虫。鞘翅目是昆虫纲中最大的类群,其种群鉴定工作复杂而艰巨。为了鉴定形态相似的鞘翅目昆虫的幼虫,本研究利用PCR扩增技术获取青海20个常见鞘翅目幼虫mtDNA上COⅠ基因的566 bp序列,结合Blast搜索、遗传距离计算和NJ系统发育树构建对其进行分类鉴定,有效鉴定出20只供试鞘翅目幼虫属于5科9属。研究结果表明,DNA条形码技术进行种类鉴定快捷、准确,mtDNA COⅠ基因能够用于鞘翅目昆虫分类鉴定。
The identification of many insect species and especially pupae and larval stages can be very diffi- cult by traditional morphological methods as a consequence of high morphological variability within species and due to the existence of sibling species. Coleopteran is the most diverse order of insects and its identifi- cation is more difficult and complex. In this study, the PCR techniques was used to amplify 20 common coleopteran larvaes in Qinghai and 566 bp sequences of Mitochondrial Cytochrome Oxidase I（ CO I ） was obtained in order to identify the larvaes which have similar morphology. Sequences were blasted in the GenBank, genetic distances were calculated, and a NJ phylogenetic tree was constructed with Kimmura-2- parameter distances mode. The results showed that all the larvaes belonged to 5 families and 9 genera based on the analysis of three kinds of methods. In conclusion that DNA Barcoding is a fast and accurate tool for species identification and mtDNA CO I sequence data can be used as standard DNA barcoding marker for successful species identification of coleopteran insects.
出处
《草业科学》
CAS
CSCD
北大核心
2013年第8期1253-1258,共6页
Pratacultural Science
基金
国家自然科学基金项目(40901040
41171054)
冰冻圈科学国家重点实验室自主课题(SKLCS 09-06)
