摘要
目的 探讨人参多糖（ginseng polysaccharide，GPS）对人鼻咽癌细胞CNE-2裸鼠移植瘤的放疗增敏作用及可能的机制。方法取对数生长期的鼻咽癌CNE-2细胞，经裸鼠背部皮下注射，1×10^7个／（0.2ml·只），待瘤体最大径为4～6mm时，取20只模型裸鼠，随机分为4组：对照组（经腹腔注射生理盐水）、放疗组（每次每只裸鼠经肿瘤局部给予5GyX射线照射，每3d照射1次，共3次）、GPS组（经腹腔注射GPS，30mg／kg，每次0.3ml，每72h给药1次，共5次）和GPS联合放疗组（经腹腔注射GPS，30mg／kg，每次0.3ml，每72h给药1次，共5次；末次给药48h后开始放疗，每只每次经肿瘤局部给予5Gy X射线照射，每3d照射1次，共3次）。开始给药3周后处死裸鼠，取瘤体，称重，测量肿瘤的长、短径，计算肿瘤体积及抑制率；HE染色观察移植瘤组织病理学改变；Real-time PCR检测移植瘤组织中β-eatenin基因mRNA的转录水平；免疫组织化学和Western blot法检测移植瘤组织中β-catenin蛋白的表达水平。结果与对照组相比，GPS组、放疗组和GPS联合放疗组的肿瘤体积及瘤重均明显下降（P〈0.05），抑制率分别为29.87％、52.60％和74.68％；镜下观察可见，GPS联合放疗组细胞凋亡明显，多数细胞核完全溶解，细胞结构消失，坏死的肿瘤组织呈现均质红染表现；GPS联合放疗组移植瘤中β-cateninmRNA及蛋白的表达水平均显著下降（P均〈0.05）。结论GPS可抑制CNE-2细胞在裸鼠体内的生长，且对鼻咽癌的放疗具有增敏作用，其机制可能与抑制β-catenin的表达有关。
Objective To investigate the enhancement of radiosensitivity of xenotransplanted nasopharyngeal carcinoma CNE-2 cells in nude mice by ginseng polysaccharide as well as the relevant mechanism. Methods CNE-2 cells at logarithmic growth phase were injected s.c. into nude mice, 0. 2 ml （containing 1 × 10^7 cells） for each. Twenty model nude mice with tumors at the maximum diameters of 4 - 6 mm were randomly divided into four groups. The mice in control group were injected i.p. with physiological saline, while those in radiotherapy group were radiated with 5 Gy X ray every 3 d for 3 times, those in GPS group were injected i.p. with GPS at a dosage of 30 mg/kg in a volume of 0. 3 ml, every 72 h for 5 times, and those in GPS ＋ radiotherapy group were injected i.p. with GPS at a dosage of 30 mg/kg in a volume of 0. 3 ml, every 72 h for 5 times, then received a radiotherapy 48 h after the last injection by radiation with 5 Gy X ray, every 3 d for 3 times. All the nude mice were killed 3 weeks after the first injection, of which the tumors were weighed and measured for longitudinal and transverse diameters to calculate the tumor size and inhibition rate. The pathological change of tumor was observed by HE staining, in which the transcription level of β-catenin mRNA was determined by real-time PCR, while the expression level of β-catenin protein by Western blot. Results Both sizes and weights of tumors in GPS, radiotherapy and GPS ＋ radiotherapy groups decreased significantly as compared with those in control group （each P 〈 0. 05）, with inhibiting rates of 29.87%, 52.60% and 74.68% respectively. Obvious cell apoptosis was observed in GPS ＋ radiotherapy group, expressed as complete dissolution of most of cell nuclei and disappearance of cell structure, while the necrotic tumor cells were stained red evenly. Both the expression levels of 15-catenin mRNA and protein in GPS ＋ radiotherapy group decreased significantly （each P 〈 0. 05）. Conclusion GPS inhibited the growth of CNE-2 cells in nude mice a
出处
《中国生物制品学杂志》
CAS
CSCD
2014年第4期538-543,共6页
Chinese Journal of Biologicals
基金
国家自然科学基金(81171929
31271368)
国家自然科学基金青年基金(81001582)
关键词
人参多糖
鼻咽癌
CNE-2细胞
放疗
敏感性
Β-CATENIN
Ginseng polysaccharide （GPS）
Nasopharyngeal carcinoma
CNE-2 cells
Radiation therapy
Sensitivity
15-catenin
