摘要
该研究以甘蔗条纹花叶病毒(Sugarcane streak mosaic virus,SCSMV)的P3蛋白(SCSMV-P3)为诱饵,采用酵母双杂交技术(yeast two-hybrid system,Y2HS)从甘蔗酵母文库中筛选到1个编码核酮糖-1,5-二磷酸羧化酶/加氧酶(ribulose-1,5-bisphosphate carboxylase/oxygenase,Rubisco)大亚基的基因,命名为ScRbcL。序列分析表明,该基因开放读码框(open reading frame,ORF)长度为1 337bp,编码478个氨基酸残基。Y2HS和双分子荧光互补(bimolecular flourescence complementation,BiFC)实验表明,ScRbcL与SCSMV-P3存在互作关系。研究认为SCSMV-P3与ScRbcL的互作可能是SCSMV侵染甘蔗导致花叶症状发生的分子基础。
Sugarcane streak mosaic virus （SCSMV） is one of the causative agents of sugarcane （Saccharum officinarum L. ） mosaic disease. In this study a sugarcane cDNA library was screened with P3 from SC- SMV as bait using yeast two-hybrid system （Y2HS）. A gene encoding the large subunit of ribulose-1,5-bi- sphosphate carboxylase/oxygenase （Rubisco） was obtained and designed as ScRbcL. The open reading frame （ORF） of ScRbcL is 1 337 bp in length and codes for 478 amino acids. The interaction between the ScRbcL and SCSMV-P3 was identified by Y2HS and bimolecular fluorescence complementation （BiFC）. This research provided new cues to the investigation of the molecular mechanism of SCSMV infection on sugarcane, especially on the mosaic syndrome development caused by sugarcane mosaic disease.
出处
《西北植物学报》
CAS
CSCD
北大核心
2014年第4期676-681,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家863课题(2013AA102604)
国家自然科学基金(31171605,31371688)
