摘要
目的研究PI3K和caspase-3在食管鳞癌组织(esophageal squamous cell carcinoma,ESCC)中的表达及其与食管肿瘤发生、发展与转移的关系。方法应用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶链结(SP)法检测24例正常食管黏膜、94例食管鳞癌组织中PI3K和caspase-3的表达情况,并分析其与临床病理特征之间的关系。结果PI3K在食管鳞癌中的表达高于正常食管黏膜(P<0.01);PI3K蛋白在高-中分化鳞癌中表达低于低分化鳞癌,随着浸润深度和临床分期的增加,其表达阳性率增高,在淋巴结转移阳性组的表达高于阴性组(92.0%vs 47.7%,P<0.01);而caspase-3阳性表达与PI3K相反,高-中分化鳞癌中表达高于低分化鳞癌,随着浸润深度和临床分期的增加,其表达阳性率降低,在淋巴结转移阳性组的表达低于阴性组(38.0%vs 68.2%,P<0.01);PI3K与caspase-3表达有关联(r=-0.230,P<0.05)。结论 PI3K高表达和caspase-3低表达与食管鳞癌发生发展及侵袭转移存在协同作用,是反映食管鳞癌生物学行为的重要指标。
Objective To study the expression of PI3K and caspase-3 in esophageal carcinoma for the relationship between the expression of PI3K and caspase-3 as well as carcinogenesis or progression of esophageal carcinoma.Methods PI3K and caspase-3 expression were detected in 24 normal esophageal mucosa,94 primary tumor tissues with SP immunohistochemical method.Results The expression of PI3K in esophageal carcinoma was higher than that of normal mucosa epithelium（P 〈0.01）.PI3K expression in higher-differentiated group was lower than that in low-differentiated group.With invasive depth and clinical staging aged,its expression became higher,so did it with metastasis of lymph node （92.0% vs 47.7%,P 〈0.01 ）.But the positive expression rate of caspase-3 was on the contrary.In higher-differentiated group,its positive expression rate was higher than in low-differentiated group.With invasive depth and clinical staging aged,its expression tended lower.In carcinoma with lymph node metastasis,it showed lower expression than in that of lymph node non-metastasis （38.0% vs 68.2%,P 〉 0.01 ）.There was correlation between PI3K and caspase-3 expression in this study （r = -0.230,P 〈 0.05 ）.Conclusion PI3K and caspase-3 are over expressed in esophageal carcinoma.They play important roles in carcinogenesis and progression of＆amp;nbsp;esophageal carcinoma and can be used as valuable biomarkers to evaluate biological characteristics in esophageal carcinoma.
出处
《临床荟萃》
CAS
2014年第5期546-548,共3页
Clinical Focus
基金
河北省卫生厅医学科研基金(20110136)
