摘要
目的:探讨HMGB1和RAGE蛋白在胃癌组织中的表达及其与临床病理因素的相关性。方法:收集2005-01-01-2008-12-30苏州市立医院手术切除的240例胃癌标本及同期患者40例正常胃黏膜组织(距离癌组织≥7cm),制成组织芯片蜡块,免疫组织化学法检测石蜡包埋的正常胃黏膜及胃癌组织HMGB1及RAGE蛋白表达。多因素分析采用Cox回归模型,HMGB1和RAGE蛋白表达的相关性采用Spearman等级相关分析法。结果:正常胃黏膜组织中HMGB1和RAGE蛋白的表达率分别为17.5%和20.0%,且均为弱阳性;胃癌组织中阳性表达率分别为43.8%和44.6%;HMGB1和RAGE蛋白在正常胃黏膜组织和胃癌组织中的阳性表达比较,差异有统计学意义,P<0.01。HMGB1表达与肿瘤TNM分期(χ2=4.911,P=0.027)和淋巴结转移(χ2=3.893,P=0.048)相关;RAGE表达仅与淋巴结转移(χ2=4.887,P=0.027)相关;两蛋白表达与患者年龄、性别、肿瘤分化程度以及浸润深度无关,P>0.05。多因素分析结果显示,淋巴结转移是HMGB1蛋白表达的独立因素。HMGB1及RAGE蛋白表达呈正相关,r=0.138,P=0.032。结论:HMGB1和RAGE蛋白可能是预测胃癌组织浸润和转移的有用指标;联合检测HMGB1和RAGE蛋白可作为判断胃癌预后和筛选高危转移患者的有效标志,并有可能用于指导胃癌的个体化治疗。
OBJECTIVE：To investigate the HMGB1 and RAGE protein expression in gastric cancer tissues and study the correlation between the HMGB1-RAGE axis protein expression and the relation of metastatic activity in gastric carcinomas tissue. METHODS：Immunohistochemistry was used to detect the HMGB1 and RAGE protein expression in paraffin-embedded normal gastric mueosa tissues and gastric cancer tissue samples in tissue microarray（TMA） in Suzhou Mu- nicipal Hospital from Jan. 1,2005 to Dee. 30,2008. Another 40 paraffin-embedded tissue blocks of normal gastric epithelium （〉7 cm away from the edge of the tumor） were selected as the control group. Cox hazard regression model were employed to make multivariate analysis. The relationship between the HMGB1 and RAGE protein expression was treated by Spearman correlation analysis. RESULTS： Among the 40 cases of normal gastric epithelium, the expression rate of the HMGB1 and RAGE protein was 17. 5% and 20. 0%. Among 240 cases of gastric cancer, the expression rate of the HMGB1 and RAGE protein was 43.8% and 44.6% respectively. The HMGB1 and RAGE protein expression in gastric cancer were significantly higher than that in normal gastric mucosa（P〈0.01）. There was a close relationship between the HMGB1 and RAGE protein expression with lymph node metastasis （X2 = 3. 893, P= 0. 048;X2 = 4. 887, P= 0. 027）, the HMGB1 protein expression had statistically significant correlation with TNM stage （X2 =4. 911 ,P=0. 027）. Both of them had no statistically significant correlation with age, gender, tumor differentiation degree and the depth of tumor invasion （P〉0.05）. Multivariate analysis showed lymph node metastasis was an independent factor influencing the expression of HMGB1 protein. The expression of HMGB1 was positively correlated to that of RAGE in gastric cancer（r= 0. 138,P= 0. 032）. CONCLUSIONS： H MGB1 and RAGE protein can be useful markers to predict gastric cancer invasion and metastasis process. The expression of the HMGB1 and RAGE protein combi
出处
《中华肿瘤防治杂志》
CAS
北大核心
2014年第9期684-687,691,共5页
Chinese Journal of Cancer Prevention and Treatment
基金
江苏省教研室课题资助项目(j201203)
关键词
胃肿瘤
高迁移率族蛋白B1
晚期糖基化终末产物受体
免疫组织化学
stomach neoplasms
high mobility group box B1
receptors for advanced glycation end-products
immunohistochemistry
