摘要
NAC(NAM-ATAF1,2-CUC2)转录因子在植物胁迫响应中起重要作用。为了探讨LeNLP4基因在番茄抗低温胁迫中的功能,分离了番茄LeNLP4转录因子基因,并获得转正义LeNLP4基因番茄植株。荧光定量PCR分析表明,LeNLP4的表达受低温诱导。与野生型植株相比,在4℃胁迫下转基因植株具有较高的生长量和光系统Ⅱ(PSⅡ)最大光化学效率(F_v/F_m)、过氧化氢(H_2O_2)和超氧阴离子(O_2)清除速率、抗坏血酸过氧化物酶(APX)和超氧化物歧化酶(SOD)活性,以及较低的丙二醛(MDA)含量和相对电导率(REC)。过表达株系中SlCBF1的表达高于野生型。上述结果表明,LeNLP4的过表达提高了转基因番茄抗低温胁迫能力。
NAC （NAM-ATAF 1,2-CUC2） family members play important roles in various environmental re- sponses. In order to investigate the function of LeNLP4 under chilling stress, LeNLP4 was isolated from tomato （Lycopersicon esculentum） and transgenic plants were obtained, qRT-PCR confirmed that the expression of LeNLP4 was induced by chilling stress. Compared with the wild type （WT）, transgenic tomato plants have higher growth increment and the maximal photochemistry efficiency ofphotosystem II （Fv/Fm）, higher capabili- ty for scavenging hydrogen peroxide （H202） and superoxide radical （O2-）, higher ascorbate peroxidase （APX） and superoxide dismutase （SOD）, and lower malondialdehyde （MDA） content and the relative electric conduc- tivity （REC） under chilling treatment. Expression level of SICBF1 in transgenic lines was higher than that in WT. These results indicated that overexpression of LeNLP4 enhanced chilling tolerance of transgenic tomato plants.
出处
《植物生理学报》
CAS
CSCD
北大核心
2014年第4期501-509,共9页
Plant Physiology Journal
基金
国家重点基础研究发展计划(2009CB118505)
国家自然科学基金(31171474和31371553)
