摘要
目的采用紫外分光光度法测定肝灵饮品中五味子醇甲进行定量检测，并对紫外分光光度法进行方法学研究。方法将肝灵饮品通过浓缩，定容，超声，稀释等步骤处理，用紫外分光光度计在254nm波长下检测五味子醇甲的含量。结果本方法在0．006～0．078mg／ml范围内线性关系良好，R^2=0．9995，回收率为98．33％-98．79％，相对标准偏差为0．34％～0．85％。结论该方法具有准确度高，精密度好，能够满足测定肝灵饮品中五味子醇甲含量的要求。
Objective To study the measurement of schizandrin in ganling by ultraviolet spectrophotometry and make a methodological observation. Methods The ganling was concentrated, constant volume, ultrasonic treatment, dilution steps, schisandrin were measured in 254 nm wavelength by UV spectrophotometer. Results This method had good correla- tion （ R^2 = 0. 999 5 ） between 0. 006 - 0. 078 mg/ml, the coefficient of recovery was 98. 33% - 98. 79% , the relative standard deviation was 0. 34% -0. 85%. Conclusion With high accuracy and precision, this method can meet the need of detecting the sehizandrin in ganling.
出处
《中国卫生工程学》
CAS
2014年第2期142-143,145,共3页
Chinese Journal of Public Health Engineering
