摘要
目的 :研究黄芩苷对体外培养大鼠肝细胞凋亡的影响。方法 :将黄芩苷作用于体外培养的大鼠肝细胞 ,以肿瘤坏死因子 (TNF α)和放线菌素D(ActD)诱导细胞凋亡 ,MTT法检测细胞活性 (A值 ) ;溴甲酚绿法检测细胞功能 (ALB值 ) ;琼脂糖凝胶电泳和流式细胞术检测法定性定量检测细胞凋亡情况。结果 :经 0 .2 ,2 .0 μg·ml-1浓度黄芩苷作用后肝细胞活性 (A值 )及分泌白蛋白功能 (培养上清中ALB值 )均高于凋亡模型组 (A值P <0 .0 5 ,ALB值P <0 .0 1) ,其中 0 .2 μg·ml-1浓度组肝细胞的ALB值比空白对照组还高 (P <0 .0 1) ;琼脂糖凝胶电泳显示 :只有凋亡模型组出现了典型的”梯状”条带 ;流式细胞术检测结果表明 :各中药组的凋亡率与凋亡模型组相比均有显著性差异 (P <0 .0 1)。结论 :不同浓度的黄芩苷均对TNF α和ActD所诱导的肝细胞凋亡有抑制作用。
Objective: To stuby the effects of baicalin on rat hepatocyte apoptosis induced by tumornecrosis factora (TNF α) and actinomycin D (Act D) in vitro. Methods: Hepatocytes were cultared with different concentrations of baicalin and the cell apoptosis was induced by TNF α and Act D in vitro. After culturing for 24 h, the activity of hepatocyts was examined by MTT assay and the function of hepatocytes for secreting albumin was detected by bromocresolum Viride method. Agarose gelelectrophoresis and flowcytometric analysis were used to determine the apoptotic cells. Results: The activety of the hepatocyte (A) and content of albumin (ALB) in the supematant of cultured cells in the groups of 0.2 μg·ml -1 and 2.0 μg·ml -1 of baicaline were significantly higher than those in apoptotic model group (A: P <0.01; ALB: P <0.01);respectively Even the ALB value in the group of 0.2 μg·ml -1 baicalin was higher that of black control group ( P <0.01); The clear “ladder like” streak band of DNA fragments was foundonly in the apoptosis model group by the agarose gel electrophoresis. The flow cytometry showed that all the apoptosis rates in the three baicalin groups were lower than that of the apoptotic model group ( P <0.01)。Conclusion: Baicaline can restrain rat hepatocyte apoptosis induced by TNF α and ActD in vitro. [
出处
《中国中药杂志》
CAS
CSCD
北大核心
2001年第2期124-127,共4页
China Journal of Chinese Materia Medica
