摘要
目的检测血管内皮生长因子(VEGF)及E-cadherin在胃癌组织中的表达,探讨其临床意义。方法采用荧光定量实时PCR定量分析病灶细胞中VEGF及E-cadherin mRNA转录量,Western blot分析VEGF及E-cadherin蛋白表达量,并采用免疫组化方法分析表达阳性率。结果胃癌组织和无癌胃黏膜组织中VEGF蛋白的阳性表达率分别为78.6%(55/70)和14.3%(5/35),E-cadherin蛋白的阳性表达率分别为22.9%(16/70)和91.4%(32/35)。VEGF mRNA在胃癌组织中的表达量高于无癌胃黏膜组织(P<0.01),E-cadherin mRNA则相反(P<0.01),且二者的表达量与肿瘤分化程度、淋巴结转移、浸润深度、TNM分期有关(P<0.05)。结论胃癌组织中VEGF mRNA和蛋白的表达增加,E-cadherin mRNA和蛋白则减少;提示VEGF和E-cadherin的表达与胃癌的生物学行为有关,可能参与胃癌的发生、发展和侵袭转移。
Objective To investigate the expression and clinical significance of vascular endothelial growth factor （VEGF） and E-cadherin in gas- tric cancer, and explore its clinical significance. Methods Real-time PCR and Western blot were used to detect the mRNA and protein expressions of VEGF and E cadherin, and immunohistochemical method was used to ＇analyze the positive rate. Results VEGF p1~otein expression rates of gas- tric cancer tissue and normal tissue were 78.6% （55/70） and 14.3% （5/35） , and the positive expression rates of E-cadhefin protein were 22.9% （ 16/70 ） and 91.4% （32/35） respectively. The expression levels of VEGF mRNA and protein were remarkably higher than those in control group （P 〈 0.01 ）, and E-cadherin was opposed （P 〈 0.01 ）. Moreover, the expression levels were correlated with minor differentiated degree, lymphatic metastasis, depth of invasion and TNM staging （P 〈 0,05 ）. Conclusion In gastric cancer, the expression levels of VEGF was obviously increased and E-cadherin was decreased. Both VEGF and E-cadherin are involved in the appearance, progress and migration of gastric cancer.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2014年第4期324-327,332,共5页
Journal of China Medical University
基金
辽宁省科学技术计划(2012225020)
