摘要
目的预测EB病毒gp125蛋白的B细胞表位。方法基于EB病毒gp125蛋白的氨基酸序列,采用亲水性参数、可及性参数、极性参数和抗原性指数方案等,辅以对gp125蛋白的二级结构中的柔性区域的分析,预测gp125蛋白的B细胞表位。结果最有可能的B细胞表位位于gp125蛋白N端第403-416、565-574、578-584、618-630和832-843区段及其附近。结论用多参数预测EB病毒gp125蛋白的B细胞表位,为制备具有高灵敏度和高特异性的鼻咽癌诊断试剂及研究抗肿瘤转移靶向治疗的分子免疫学奠定基础。
Objective To predict the B cell epitopes of gp125 protein in Epstein-Barr virus （EBV）. Methods The secondary strueture of EBV gp125 protein was predicled by the methods of GOR based on the sequence of amino acids of EBV gp125 protein. By eombining the comprehensive analysis of hydrophilicity profile, surface probability, antigenic index and average flexibility, the B cell predominant epitopes of EBV gp125 protein were further predicted. Results The most possible epitopes of EBV gp125 protein were located within or nearby its N-terminal No. 403-416, 565-574, 578-584, 618-630 and 832-843. Conclusion Prediction of the B cell epitopes of the EBV gp125 protein based on multiple parameters is helpful for the serologieal screening and target therapy of antitmnor metastasis of nasopharyngeal.
出处
《中国微生态学杂志》
CAS
CSCD
2014年第4期404-408,共5页
Chinese Journal of Microecology
基金
教育部第44批留学回国人员科研启动基金[教外司留(2012)940]
