摘要
目的:观察不同时间电针对血管性痴呆(VD)小鼠学习记忆能力及海马去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)含量的影响,探讨电针改善VD学习记忆功能障碍的可能作用机制。方法:60只昆明小鼠随机分为两批,每批各设假手术组、模型组、电针组,每组10只。采用颈总动脉结扎缺血再灌注的方法复制VD模型。第1批于造模后苏醒当日开始电针干预,第2批于造模第3天开始进行电针干预。电针组针刺"百会""大椎"及双侧"足三里""膈俞",每次10min,治疗15d。观察各组小鼠跳台学习成绩及跳台记忆成绩,荧光分光光度法检测海马NE、DA、5-HT的含量变化。结果:与假手术组比较,模型组小鼠的学习和记忆成绩均明显下降(P<0.01),海马NE、DA、5-HT含量均显著降低(P<0.01);与模型组比较,电针组小鼠学习和记忆成绩均明显提高(P<0.01),海马NE、DA、5-HT含量均显著升高(P<0.01),且造模第3天电针组优于造模当天电针组(P<0.05,P<0.01)。结论:电针能够改善VD学习记忆功能障碍,其作用机制与改善海马中单胺类神经递质代谢紊乱有关,并初步发现介入干预时间不同,其治疗效应存在差异。
Objective To observe the effect of electrocupuncture （EA） intervention at different time-points of post-mo- deling on behavior and hippocampal monoamine neurotransmitter noradrenalin （NE）, dopamine （DA） and 5-hydroxytrypamine （5-HT） contents in vascular dementia （VD） mice, so as to study its mechanism underlying improvement of VD. Methods A to- tal of 60 Kunming mice were randomized into sham-operation control （n = 20）, VD model （n = 20）, EA-day （D）-I （EA treatment was given from the 1st day on after modeling, n = 10）, EA-D-3 （EA was given from the 3rd day on after modeling, n = 10） groups. VD model was established by occlusion of the bilateral cervical common arteries and reperfusion. EA （2 Hz/80 Hz） was applied to ＂Baihui＂ （GV 20）, ＂Dazhui＂ （GV 14） ,＂Zusanli＂ （ST 36） and ＂Geshu＇（BL 17） for 10 min, once daily for 15 days. Hippocampal NE, DA and 5-HT contents were assayed by fluorospectrophotometry. The mouse＇s learning-memory ability was assessed by step-down tests. Results In comparison with the sham-operation control group, the learning-memory ability （marked increase of reaction time and error times, decrease of step-down latency） was apparently lowered in the model group （P〈0.01）. The hippocampal NE, DA and 5-HT contents were significantly lower in the model group than in the sham-operation group （P〈0.01）. Compared with the model group, the mice＇s learning-memory ability （marked decrease of reaction time and error times, increase of step-down latency） was significantly increased in EA intervention groups （ P〈0. 01 ）, and hippocampal NE, DA and 5-HT levels were significantly increased （ P〈0.01 ）, and the effect of EA-D-3 group was obviously better than that of the EA-Dol group （P〈0.05, P〈0.01）. Conclusion EA can improve the VD mice＇s learning-memory ability, which is closely related to its effects in up-regulating hippocampal NE, DA and 5-HT contents, and the effect of later EA interventi
出处
《针刺研究》
CAS
CSCD
北大核心
2014年第2期142-147,共6页
Acupuncture Research
基金
河北省科技厅科技攻关项目(No.042761388)
河北省中医药管理局资助项目(No.2004010
No.2006058)
关键词
电针
血管疾病
学习记忆能力
去甲肾上腺素
多巴胺
5-羟色胺
海马
Electrocupuncture
Vascular disease
Learning-memory ability
Noradrenalin
Dopamine
5-hydroxytrypamine~ Hippocampus
