摘要
以龙井43茶树为供试材料,采用水培法试验,研究了不同浓度的氟(F)胁迫对茶树叶片的抗氧化能力、膜脂过氧化以及茶树氟含量的影响。结果表明:氟胁迫促使茶树叶片的超氧化物歧化酶(SOD)活性先升高后保持相对稳定,过氧化氢酶(CAT)、过氧化物酶(POD)和抗坏血酸过氧化氢酶(APX)活性先升高后降低。氟胁迫下茶树的H202、丙二醛(MDA)以及脯氨酸(Pro)含量升高。茶树根部和地上部分氟含量均随着氟胁迫浓度的增加而增加,且各器官的氟含量(Y)与氟添加浓度(X)呈极显著正相关。富集系数和转运系数均大于1,说明茶树对氟有较强的富集能力。低浓度氟胁迫可以有效刺激茶树体内抗氧化酶活力,而高浓度氟胁迫下茶树叶片的抗氧化防御系统不能清除过多的活性氧化物。
With the water planning experiment, effects of fluorine stress on antioxidant abilities, membrane lipid peroxidation of leaves and the content of fluorine in tea plant （ Longjing 43） were studied. The results showed that CAT, POD and APX activities of leaves were all first increased then decreased. The SOD activities were all first increased then remained relatively stable. The content of malondialdehyde, hydrogen peroxide and proline in leaves were all increased with increasing of F treatment. The content of fluorine in root and shoot were all increased by the content increased of fluorine which was a significant positive correlation with fluorine treatment. The BF （Bioaccumulation factor） and TF （Translocation factor） were all exceeded. It showed that tea had strong ability to accumulate fluorine. Low concentration of fluorine could stimulate the antioxidant enzyme activity in tea plant, however, the antioxidant defense system of leaves in tea could not eliminate overmuch activating oxide by high concentration of fluorine stress.
出处
《核农学报》
CAS
CSCD
北大核心
2014年第4期742-747,共6页
Journal of Nuclear Agricultural Sciences
基金
现代农业(茶叶)产业技术体系建设专项资金(农科教发[2011]3号)
安徽省教育厅重点项目(KJ2011A105)
关键词
氟胁迫
茶树
抗氧化酶
生化指标
吸收累积特性
Fluorine stress
Tea plant
Antioxidant enzymes
Biochemical criterion
Absorption and accumulation
