摘要
目的:利用COI序列建立统一的鹿类药材分子鉴定方法。方法:对鹿茸、鹿角、鹿鞭、鹿筋、鹿尾、鹿胎分别进行DNA提取、COI序列扩增和序列测定,构建鹿类药材COI序列数据库,并对市售鹿类药材进行调查分析。结果:所有鹿类药材均可以使用COI通用引物进行PCR扩增和测序;鹿类药材COI序列数据库包含8个物种101份样品,物种之间相互区分明显;市售40份药材中18种为药典规定物种,22种为非药典规定物种。结论:COI序列的DNA条形码分子鉴定方法可以作为鹿类药材的统一鉴定方法,并为市售鹿类药材的鉴定提供科学依据。
Objective： Using the COI Barcode to establish the standard method to identify the traditional Chinese medicine of deer products. Methods： In this study, DNA extraction, PCR amplification, and sequencing of experimen-tal samples （deer antler, penis and testis, tendon, tail, bone, foetus） were successful. COI sequence database were constructed and commercial crude drugs of deer were investigated and analyzed. Results： By using universal COI primer,PCR amplification is preferably. All the species could be identified based on COI sequences database of tra-ditional Chinese medicine of deer which contained 101 samples of 8 species. We have collected 40 commercial crude drugs in which 18 samples were identified as species described in Chinese pharmacopoeia. Conclusion： DNA barcode technology based on COI sequence could be a standard approach to identify traditional Chinese medicine of deer products, and provide the basis for the identification of commercial deer medicine.
出处
《世界科学技术-中医药现代化》
北大核心
2014年第2期274-278,共5页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
吉林省财政厅高等教育专项(2050205):药用动物DNA条形码鉴定技术
负责人:张辉
关键词
鹿类药材
COI序列
DNA条形码
鉴定
Traditional Chinese medicine of deers, COI barcode sequence, DNA barcoding, identification
