摘要
分别从中药成分分析、活性筛选和代谢组学三方面对质谱技术在中药研究中的应用进展进行了全面综述.在中药成分分析方面,重点介绍了寡糖异构体的分析方法,以及质谱指纹图谱技术在中药成分分析及质量控制中的应用;在活性筛选方面,分别介绍了超滤-质谱、细胞膜色谱-质谱、微透析-质谱、亲和色谱-质谱、强度衰减质谱、修饰琼脂糖珠-质谱和直接分析质谱等技术及其应用;在代谢组学研究方面,对中药治疗肝损伤、肾虚、心肌梗死和糖尿病等疾病方面的研究进展进行了阐述.上述内容充分反映了质谱技术在中药创新性研究中的重要性.
The paper presented the application advance of mass spectrometry techniques on Chinese medicine research from three aspects： the component analysis, bio-activity screening and metabonomics research. The section of the component analysis focused on various methods for analyzing oligosaccharide isomers. And the applications of the fingerprint technique of mass spectrometry to the component analysis and quality control of Chinese medicine were also provided. On the research of bioactivity compound screening, some techniques were introduced respectively, including the ultrafiltration-mass spectrometry, cell membrane chromatography-mass spectrometry, microdialysis probe-mass spectrometry, affinity chromatography-mass spectrometry, intensity-fading mass spectrometry, modified agarose-mass spectrometry and direct analysis mass spectrometry, etc. On the metabonomics studies, we provided some examples which clarified the action mechanism of Chinese medicine on hepatotoxicity, kidney deficiency, myocardial infarction and type 2 diabetes. The above content reflected the importance of mass spectrometric techniques in the innovative research of Chinese medicine.
出处
《中国科学：化学》
CSCD
北大核心
2014年第5期701-709,共9页
SCIENTIA SINICA Chimica
基金
国家自然科学基金(21175128,81373952,81073040,81373952,21073178)
国家重点基础研究发展计划(2011CB505300,2011CB505305)
科技部创新方法专项项目(2012IM030600)资助
关键词
中药
成分分析
活性筛选
生物靶分子
代谢组学
Chinese medicine, component analysis, bioactive compound screening, bio-target molecule,metabonomics
