摘要
本文建立了解吸附电晕束电离质谱(DCBI-MS)快速检测保健食品和中成药中违禁添加的9种β2-受体激动剂的分析方法。采用DCBI源正离子模式在全扫描模式下进行扫描,考察了温度和进样体积对解吸附效率的影响,9种β2-受体激动剂样品的快速测定不受基质影响,各目标化合物在1 min内均可被检出。基于二级特征碎片离子的定量分析结果表明,9种β2-受体激动剂在1～100 mg.L 1内呈线性,相关系数(r2)均大于0.991 7。相对标准偏差在14.29%～25.13%之间。利用该方法对10种市售标具止咳平喘作用的保健品及中成药进行检测,结果均为阴性。本方法无需复杂的前处理过程,简便、快速、灵敏度高,可用于止咳平喘类保健食品及中成药中违禁添加的β2-受体激动剂的快速定性及半定量检测。
A novel rapid method for detection of the illicit β2-agonist additives in health foods and traditional Chinese patent medicines was developed with the desorption corona beam ionization mass spectrometry（DCBI-MS） technique.The DCBI conditions including temperature and sample volume were optimized according to the resulting mass spectra intensity.Matrix effect on 9 β2-agonists additives was not significant in the proposed rapid determination procedure.All of the 9 target molecules were detected within 1 min.Quantification was achieved based on the typical fragment ion in MS2 spectra of each analyte.The method showed good linear coefficients in the range of 1 100 mg.L 1 for all analytes.The relative deviation values were between 14.29% and 25.13%.Ten claimed antitussive and antiasthmatic health foods and traditional Chinese patent medicines from local pharmacies were analyzed.All of them were negative with the proposed DCBI-MS method.Without tedious sample pretreatments,the developed DCBI-MS is simple,rapid and sensitive for rapid qualification and semi-quantification of the illicit β2-agonist additives in health foods and traditional Chinese patent medicines.
出处
《药学学报》
CAS
CSCD
北大核心
2013年第1期113-118,共6页
Acta Pharmaceutica Sinica
基金
国家"863"项目(2010AA023001)
国家自然科学基金资助项目(20927005
21275049)
湖南省科技计划项目(2010TT1001)
