摘要
再生水回用是解决城市水危机的重要举措,但使用再生水对土壤和土壤微生物的影响研究有限。本研究以自来水灌溉为对照,采用多种土壤指标测定法和微生物稀释涂布分离法研究了再生水灌溉对土壤理化性质和微生物群落多样性的影响。结果表明:经再生水灌溉,土壤pH、含水量、有机质(OM)、全氮(TN)、全钾(TK)、碱解氮(AN)和速效磷(AP)含量增加,其中TK的影响显著,而土壤全磷(TP)、碳氮比(C/N)和速效钾(AK)值均降低;从研究区土壤分离出62株细菌划分为20个种属,其中芽孢杆菌属(Bacillus)是再生水灌区土壤细菌的优势菌属,假单胞菌属(Pseudomona)是自来水灌区的优势菌属;不同水源灌溉下微生物的多样性水平存在差异,再生水灌区土壤微生物均匀度指数(Jsw)和种间相遇机率(PIE)高于自来水灌区,而多样性指数(H')、均匀度指数(Jsi)、丰富度指数R1和R2均低于自来水灌区;多样性指数、丰富度指数和均匀度指数之间的密切关系可以反映微生物群落特征具有一致性。微生物类群聚类分析结果表明,微生物被划分为4个类群,再生水灌区(RW)和自来水灌区(DW)相似性最低,而再生水灌区内曝气池处(RW-B)和进水口处(RW-J)相似性最高。通过对再生水灌区微生物群落与土壤理化指标的典型对应分析发现,OM、TN、TP 3个指标与微生物群落相关性较强。OM对土壤杆菌属(Agrobacterium)和奈瑟氏球菌属(Neisseria)的影响较大,TP对黄单胞菌属(Xanthomonas)、纤维单胞菌属(Cellulomonas)、默氏利斯特氏菌(Listeria murrayi)和缠绕棒杆菌(Corynebacterium fascians)影响较大。
The reuse of reclaimed water is a significant measure to solve the urban water crisis, but few studies have examined the effect of reclaimed water on the soil and soil microorganisms. Taking irrigation with tap water as the control, the effect of reclaimed water irrigation on soil physicochemical properties and microbial community diversity was studied by a variety of soil in- dex measurement and microbial dilution separation. The results showed that the soil pH, soil moisture, organic matter （OM）, total nitrogen （TN）, total potassium （TK）, available nitrogen （AN） and available phosphorus （AP） contents all showed a tendency of increase, especially TK, while the values of soil total phosphorus （TP）, carbon/nitrogen （C/N） and available potas- sium （AK） were lower under irrigation with reclaimed water than under the control treatment. A total of 62 strains of bacteria were separated from sampling sites, which belong to 20 species （genera）. Bacillus was the dominant group in the soil irrigated with reclaimed water while Pseud- omonas was dominant in the control treatment. Differences in the level of microbial diversity exis-ted between the differently irrigated soils. Soil microbial evenness index （Jsw） and the probability of interspecific encounter （PIE） were higher, while Shannon index （H＇）, Pielou evenness index （ Jsi ）, Margalef richness index （R1 ） and Patrick richness index （ R2 ） were lower in the soil irri- gated with reclaimed water. By correlation analysis, the close relationship among diversity index, richness index and evenness index reflected the consistency of microbial community characteris- tics. By hierarchical scaling analysis, the spatial pattern of the microbial community was de- scribed. The microbial community was separated into four clusters ： reclaimed water-B （ RW-B）, reclaimed water-J （RW-J）, reclaimed water-C （RW-C） and tap water （DW）. RW-B, RW-J and RW-C were of the lowest similarity, while RW-B and RW-J shared
出处
《生态学杂志》
CAS
CSCD
北大核心
2014年第5期1304-1311,共8页
Chinese Journal of Ecology
基金
黑龙江省教育厅科学技术研究重点项目(12521z010)
国家自然科学基金项目(51108145)资助
关键词
再生水
微生物
多样性
理化性质
reclaimed water
microorganism
diversity
physical and chemical properties.
