摘要
目的探讨β-细辛醚对Aβ25-35所致PC12细胞凋亡的抑制作用。方法以Aβ25-35建造体外PC12细胞损伤模型,观察β-细辛醚对其细胞存活率、细胞形态、乳酸脱氢酶(LDH)的含量和Caspase-3、Caspase-6、Caspase-9 mRNA表达的影响。结果 10μmol/L的Aβ25-35与PC12细胞共培养24 h出现明显损伤,细胞活力下降,培养液中LDH含量增多,Caspase-3、Caspase-6、Caspase-9 mRNA表达水平增高。15 mg/L、30 mg/L、60 mg/Lβ-细辛醚可有效改善Aβ25-35引起的PC12细胞形态改变,提高细胞存活力,减少LDH渗漏,明显降低Aβ25-35引起的PC12细胞Caspase-3、Caspase-6、Caspase-9 mRNA表达水平。结论β-细辛醚对Aβ25-35所致PC12细胞损伤有保护作用。
Objective It is to approach the protective effect of β-asarone on PC12 cells apoptosis induced by β-amyloid protein （Aβ25-35）.Methods The model of apoptosis of PC12 cells were induced by Aβ25-35 in vitro, the rate of cell viability, the morphological change of PC 12, the consistence of lactate dehydrogenase （LDH）, and the expression of Caspase-3, -6, -9 mRNA were observed for assessing the effect on β-asarone in PC12 cells induced by Aβ25-35 .Results Cell survival decrea-sing, LDH leakage and Caspase -3, -6, -9 mRNA expression increasing were happened in PC 12 cells cultured with 10 umol/L pre aggregated Aβ25-35 for 24 hour.15mg/L, 30 mg/L, 60 mg/L β-asarone could increase cell survival , and de-crease the level of lactate dehydrogenase and the expression of Caspase -3, -6, -9 mRNA.Conclusion β-asarone can significantly prevent PC 12 cells apoptosis induced by Aβ25-35 .
出处
《现代中西医结合杂志》
CAS
2014年第12期1282-1284,共3页
Modern Journal of Integrated Traditional Chinese and Western Medicine
