摘要
目的研究五味子乙素(SchB)对中波紫外线(ultraviolet radiation b,UVB)辐射诱导人表皮角质永生化细胞(HaCat)凋亡的抑制及其作用。方法 MTT法检测五味子乙素(SchB)对UVB辐射诱导HaCat细胞凋亡的抑制作用;Hoechst染色检测细胞凋亡;RT-PCR检测SchB对HaCaT基因表达的影响。结果 SchB对HaCaT细胞生长未显示出抑制作用,且可以抑制由UVB辐射引起的细胞凋亡,并降低了UVB诱导的p53、p21、Caspase-3等与凋亡有关基因的表达。结论 SchB可通过降低紫外线辐射后细胞的凋亡基因的表达从而抑制UVB对皮肤细胞的损伤,发挥其抗光老化作用。
Aim To investigate the inhibitory effect of schizandrin B （SchB） on ultraviolet radiation b （UVB） radiation-induced apoptosis of HaCaT ceils. Methods Methyl thiazolyl tetrazolium （MTT） assay was used to examine the effect of SchB on cell viability recovery. Cell apoptosis and necrosis were measured by Ho- chest33342 staining. The p53, p21 and Caspase-3 mRNA expressions were examined by RT-PCR. ResultsIn this study, we found that Sch B attenuated UVB-in- duced toxicity in HaCaT cells. Through Hoechst 33342 stain, we visualized that SchB could inhibit UVB-in- duced HaCaT cell death. The result demonstrated that p53, p21 and Caspase-3 mRNA levels decreased com- pared with the control group. Conclusions Sch B at-tenuates the UVB-induced toxicity of HaCaT by inhibi- ting apoptotic gene expression. It plays a role in anti- photoaging.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2014年第4期523-527,共5页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81072964)
