摘要
目的:研究人节律基因Bmal1对胃癌细胞增殖的影响及对相关基因表达的影响。方法:以RNA干扰特异沉默Bmal1的胃癌细胞BGC823作为实验组,以正常BGC823细胞为对照组;采用MTT检测Bmal1干扰前后对胃癌细胞生长的影响;采用实时定量RT-PCR检测两组细胞p53、c-Fos和c-Jun基因的表达。结果:MTT结果显示与对照组相比,6、12和24 h后实验组增殖率分别为5.78%(P=0.001)、9.20%(P=0.00)和83.08%(P=0.00),同时发现p53较对照组表达显著减少(P=0.09),与对照组比较有统计学差异,而c-Fos和c-Jun增高,但与对照组比较无显著差异(P值分别为0.125和0.269)。结论:节律基因Bmal1可能通过p53诱导胃癌增殖异常,可作为调节胃癌治疗敏感性的新靶点。
Objective To investigate the effect of Bmall on proliferation in gastric cancer cells and the molecular mechanism, and to provide theoretical and experimental basis for further research targeting circadian therapy for gastric cancer. Methods Applying RNAi technique to silence Bmall gene in BGC823 was regarded as experimental group. The normal BGC823 was as control group. The inhibitory effect of the cell line was measured by MTY assay. The mRNA expression of p53, c-Jun and c-Fos was evaluated by real-time RT-PCR. Results Comparing with the data of control group,the inhibitive rates of cell growth in experimental group after 6 h, 12 h,24 h were 5.78% （P = 0.001 ）, 9.20% （P = 0.00） and 83.08% （P= 0.00） respectively. Down-regulation of Bmall decreased p53 （P 〈 0.05）, while increased c-Fos and c-Jun expression （P 〉 0.05）. Conclusion RNAi targeting Bmall has effects on gastric cancer proliferation by down-regulating p53 mRNA.
出处
《实用医学杂志》
CAS
北大核心
2014年第7期1063-1065,共3页
The Journal of Practical Medicine
基金
湖北省卫生厅青年科技人才项目(编号:QJX2012-08)
中央财政专项经费项目(编号:2012QN050)
关键词
胃肿瘤
节律基因
增殖
Stomach neoplasms
Circadian gene
Proliferation
