摘要
[目的]对猪伪狂犬病毒野毒进行检测。[方法]根据已发表的猪伪狂犬病病毒gE基因核苷酸序列,设计合成1对引物,扩增片段长度为276 bp,优化PCR反应条件,建立检测PRV野毒的PCR方法。[结果]利用建立的PCR方法检测疑似PRV野毒感染的临床送检组织病料34份,阳性样品18份,阳性率达52.94%(18/34),选取6份阳性病料PCR产物送生工生物工程(上海)股份有限公司进行序列鉴定,测序结果表明均为PRV gE基因特异性序列。[结论]该方法敏感、特异、可靠,可用于PRV野毒感染的快速诊断和流行病学调查。
[ Objective ] To detect porcine pseudorabies virus. [ Method] Based on published pseudorabies virus gE gene sequence, designed and synthesized one pairs of primers, amplified fragment length was 276 bp, after optimization of PCR reaction conditions, established a PCR method for detection of PRV wild strain virus. [ Result ] By using PCR method,34 suspected PRV wild strain virus were detected,positive samples 18, positive rate up to 52.94% （18/34） ,6 positive samples of PC R products were identified by Shenggong Biology Engineering （ Shanghai ） Co. Ltd. , the sequencing results indicated they are all PRV gE gene specificity sequence. [ Conclusion] The method is sensitive,specific and reliable,which can be used for rapid diagnosis and epidemiologieal investigation of PRV wild virus infection.
出处
《安徽农业科学》
CAS
2014年第9期2548-2549,2553,共3页
Journal of Anhui Agricultural Sciences
