摘要
目的：探讨人参皂甙Rgl对体外与Ti微粒共培养的成骨细胞的影响及可能的干预作用。方法：体外培养新生大鼠成骨细胞，分别加入常规培养基、含胎牛血清的Ti微粒、Ti微粒＋不同剂量人参皂甙Rgl的混合液培养。酶联免疫吸附法检测成骨细胞PGE-2、TNF-α、IL-1、IL-lra、IL-6的浓度；实时荧光定量PCR法检测COX-2、TNF-α mRNA表达；WESTRENBLOT法检测COX-2蛋白表达。结果：与Ti微粒共培养的成骨细胞给予人参皂甙Rgl干预后，细胞培养液中的PGE-2、TNF-α、IL-1、IL-6含量均明显低于单纯Ti微粒组，但高于正常对照组，成骨细胞的COX-2、TNF-α mRNA表达以及COX-2蛋白表达量也呈现同样的变化趋势。结论：人参皂甙Rgl能降低Ti微粒诱导的成骨细胞炎性细胞因子含量，对假体松动具有一定防治作用。
Objective：To explore the effect of ginsenosides Rgl with Ti particles on osteoblasts cultured in vitro and the pos-sible way of intervention. Methods：Osteoblasts from neonatal rats were cultured in vitro with conventional medium, fetal bovine serum with Ti particles or Ti particles along with different doses of Rgl. PGE-2, TNF-α, IL-1, IL-lra, IL-6, COX-2, TNF-α, mRNA, COX-2 protien in those three medium were detected by Enzyme linked immunosorbent assay, Re-al time fluorescence quantitative PCR method and WESTREN BLOT method respectively. Results：The level of COX-2, PGE-2, TNF-α, IL-1, and IL-6 in the Ti particles along with different doses of Rgl medium and COX-2, TNF-α mRNA COX-2 protien in cells of this group were higher than those of control group but lower than those of fetal bovine serum with Ti particles group. Conclusion：ginsenosides can reduce the levels of inflammatory cytokines produced by osteoblasts on in-duction with Ti particles and can prevent prosthesis loosening.
出处
《中国中医骨伤科杂志》
CAS
2014年第4期1-5,共5页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
国家自然科学基金资助项目（81173282）福建省科技厅重点项目（2009Y0028）教育部博士点基金（200803930001）
关键词
成骨细胞
人参皂甙
Ti微粒
假体松动
炎症因子
Osteoblasts
Ginsenosides
Ti particles
Prosthesis loosening
Inflammatory factors
