摘要
目的探讨动脉粥样硬化(atherosclerosis,AS)患者雌激素受体α(estrogen receptorα,ER-α)基因启动序列CpG岛的甲基化改变情况。方法经颈部血管彩超检查共筛选出72例AS患者,健康对照者30例。采集所有研究对象的外周血,巢式甲基化特异性PCR(nested-methylation-specific PCR,nMSP)法检测ER-α基因启动子区CpG岛的甲基化状态,对检测结果进行统计学分析。结果 72例AS患者,ER-α基因启动序列有49例发生了甲基化,其中,完全甲基化22例,半甲基化27例,甲基化率为68.06%(49/72);30例健康对照者ER-α基因启动序列有9例发生了甲基化,其中完全甲基化2例,半甲基化7例,甲基化率为30.00%(9/30),两组甲基化率差异具有统计学意义(P<0.01)。将AS患者分为未甲基化组、半甲基化组和完全甲基化组,随甲基化程度的增高,Crouse积分与颈动脉内膜中层厚度(carotid intima-media thickness,CIMT)均显著增高,组间差异均有统计学意义(P均<0.01),不同甲基化组间两两比较,差异亦均有统计学意义(P均<0.01)。ER-α基因启动序列甲基化程度与Crouse积分和CIMT间呈明显正相关,相关系数r分别为0.696和0.712(P均<0.01)。结论 AS患者ER-α基因启动序列CpG岛高度甲基化,且甲基化程度与AS程度呈正相关,ER-α基因的高甲基化很可能促进AS的发生、发展。
Objective To investigate the methylation status in promoter region of estrogen receptor a （ER-a） gene in atherosclerosis （AS） patients. Methods 102 cases including 72 AS patients and 30 healthy control subjects were selected under carotid colorized uhrasound examination. Peripheral blood samples were prepared from all subjects. The methylation status of CpG islands in ER-a gene promoter region were analyzed by nested-methylation-specific PCR（nMSP）. The results were analyzed statistically. Results Hypermethylation of ER-a gene promoter region was found in 49 cases of 72 AS patients, 22 cases were full-methylation and 27 eases were half-methylation, the methylation frequency was 68.06% （49/72）. While in 30 healthy control subjects, only 7 cases were half-methylation and 2 cases were full-methylation, the methylation frequency was 30.00% （9/30）, which much lower than the one in AS patients and the difference had statistical significance （P〈 0.01 ）. The Crouse integral and carotid intima-media thickness （CIMT） showed an upward trend along with the increase of methylation degree, and the differences among three groups all had statistical significance （Pall〈 0.01 ）. The difference between each two methylation groups all had statistical significance （Pall〈 0.01 ）. The Spearman rank correlation analysis explored an obviously correlation between the degree of methylation in ER-a gene promoter region and the severity of AS lesion. The coefficient correlation r were 0.696 and 0.712 respectively（Pall〈 0.01 ）. Conclusion Hypermethylation of CpG islands in ER-a gene promoter region was found in AS patients, which is positive correlated with the severity of AS lesion. The hypermethylation of ER-a gene hence to promote the occurrence and development of AS.
出处
《实用检验医师杂志》
2013年第4期210-214,共5页
Chinese Journal of Clinical Pathologist
