摘要
With Biolog Eco microplate, metabolic characteristics and functional diver-sity of carbon source in microflora of ponds were researched based on recitculating ponds and control ponds in order to explore effects of eco-adjustments on microflo-ra in ponds. The results indicate that total number of bacterium, microbial metabolism activity, and diversity index in P7, P8, P1 and P2 kept higher, fol owed by P3, P4, P5 and P6. The utilization rate of microbes on sugars achieved the highest （31.0%-48.7%）, fol owed by carboxylic acid （13.4%-18.0%）, amino acid （10.1%-20.5%）, polymers （9.4%-17.0%）, biopolymer （5.7%-9.7%） and phenol （4.95%-7.50%）. Principal component analysis divided microflora in different ponds, suggesting that microbial community has varied carbon source characteristics and nitrogen-containing compound and biopolymer metabolisms are most affected.
利用Biolog Eco微板技术,以循环水养殖池塘和对照塘为实验对象,研究了池塘水环境中微生物群落对碳源利用的代谢特性和功能多样性,来探讨生态调控措施对养殖池塘微生物群落的影响。结果表明,对照塘P7、P8和循环塘P1、P2细菌总数、微生物代谢活性、多样性指数较其他循环塘高,其次是P3、P4、P5,P6是最低的。各池塘微生物对糖类的利用率最高(31.0%～48.7%),其次为羧酸(13.4%～18.0%)、氨基酸(10.1%～20.5%)和聚合物(9.4%～17.0%),胺类和酚类的平均利用率分别为5.7%～9.7%、4.95%～7.5%。主成分分析将不同池塘的微生物群落很好的分离开来,这说明,不同池塘的微生物群落有着不同的碳源代谢特性,受影响最大的是含氮化合物和聚合物的代谢。
基金
Supported by National Modern Agricultural Technology System(CARS-46)
NationalSci-tech Support Plan(2012BAD25B05,2012BAD25B01)
National Department PublicBenefit Research Foundation(201203083)~~
