摘要
目的诊断导致树鼩(Tupaia belangeri)腹泻的肠道致病菌,为疾病预防和控制提供参考。方法采集6只腹泻树鼩新鲜粪便,按常规肠道细菌鉴定方法进行分离培养,观察细菌形态和各菌种比例,对优势可疑菌进行生化和分子生物学鉴定,并进行了8种常用抗菌素的敏感试验。结果培养菌落在营养琼脂培养基上多数呈黄白色,表面光滑湿润;在S.S琼脂培养基上菌落为圆形、中心发黑,有的蔓延成黑色波纹状且有臭味。细菌形态为两端钝圆短杆菌,大小为(0.4—0.6μm)×(1.0—3.0μm),无芽孢和荚膜,革兰氏染色阴性,非抗酸菌。生化鉴定该分离株产硫化氢,鸟氨酸、尿素等反应阳性。核酸扩增后与GenBank中奇异变形杆菌相似度达99%。鉴定为奇异变形杆菌。该分离株对左氧氟沙星、环丙沙星等8种药物均敏感。结论作为条件致病菌的奇异变形杆菌能够引起哺乳期母树鼩和仔树鼩腹泻甚至死亡。实施针对性的饲养管理和药物治疗,可有效地控制该疾病的传播。
To identify the pathogen from diarrheic tree shrews which may provide an useful reference for preventing and treatment diseases in tree shrews （Tupaia belangeri Chinensis）.6 fresh feces were collected,and identified the dominant and suspicious bacteria by cultural characteristics,colony morphology,staining,biochemical testing and molecular biology.Antibiotic sensitivity of isolated strains were detected with 8 kinds of antibiotics.Most colonies were white-yellow with smooth and damp surface on nutrient agar plates,round with dark cores.Some of them spread black ripples and smelly odour.Bacterial character were brevibacterium with blunt,without spore and capsule,size were （0.4 ～0.6 μm） × （1.0 ～3.0 μm）,gram negative and non acid fast bacteria.The isolated strain produced hydrogen sulfide.The reactions of ornithine,urea etc were positive.Genetic similarity were 99% with the Proteus mirabilis from GenBank.All results showed the isolated was a strain of Proteus mirabilis.The isolated strains were sensitive to all tested antibiotics.The Proteus mirabilis isolated from diarrheic tree shrews is the pathogenic agent leading to diarrhea and death in lactation tree shrews and infant.It was be controlled by improving management and treatment.
出处
《实验动物科学》
2014年第1期41-44,共4页
Laboratory Animal Science
基金
国家科技支撑计划(2011BAI15B01-21
2012BAI39B01
2014BAI01B01)
云南省科技创新平台建设(2013DA002)
关键词
树鼩
奇异变形杆菌
分离鉴定
药敏试验
tree shrews
Proteus mirabilis
isolation and identification
antibiotic sensitivity test
