摘要
目的掌握辽宁省疟疾传播媒介雷氏按蚊的构成和分布,为制定媒介按蚊控制策略提供科学依据。方法利用形态学和分子生物学方法对2010年在辽宁省沈阳、大连、丹东、锦州和铁岭市等不同地理环境采集到的按蚊进行种类鉴定。结果分子生物学鉴定结果显示,沈阳、大连、丹东、锦州和铁岭市雷氏按蚊物种构成比分别为26%、66%、73%、33%和83%,铁岭最高,沈阳最低;形态学鉴定显示,沈阳、大连、丹东、锦州和铁岭市雷氏按蚊物种构成比分别为30%、74%、80%、33%和72%;5个市PCR鉴定和形态学鉴定雷氏按蚊构成结果的比对,经统计学检验差异无统计学意义。结论雷氏按蚊在沈阳、大连、丹东、锦州和铁岭市均有分布,并且在大连、丹东和铁岭市为优势种;初步证实中国雷氏按蚊分布的北部边界已向北推移至北纬43°26'的铁岭市。
Objective To examine the distribution and composition of Anopheles Lesteri in Liaoning province and to provide evidence for the development of control strategy for the insect vector.Methods Morphological and molecular biological methods were used to identify Anopheles Lesteri among Anopheles samples collected in Shenyang,Dalian,Dandong,Jinzhou,and Tieling city of Liaoning province in 2010.Results The proportions of Anopheles Lesteri in Shenyang,Dalian,Dandong,Jinzhou,and Tieling were 26%,66%,73%,33%,and 83%,respectively,and the proportion was highest in Tieling and lowest in Shenyang.The results of morphological identification showed that the constituent ratio of Reye Anopheles were 30%,74%,80%,33%,and 72% for the samples collected in Shenyang,Dalian,Dandong,Jinzhou,and Tieling city,respctively,and there was no significant difference in the ratios between PCR identification and morphological identification（χ2=3.090 2,P〉0.05）.Conclusion Anopheles Lesteri was identified in all cities surveyed and was the dominant Anopheles in Dalian,Dandong and Tieling city.The north border of Anopheles Lesteri＇s distribution in China has moved to Tieling city,located in the northern latitude of 43°26＇.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2014年第4期475-477,共3页
Chinese Journal of Public Health
关键词
疟疾
雷氏按蚊
分布
辽宁省
malaria Reye Anopheles distribution Liaoning province
