摘要
目的:观察不同材质导尿管对大肠埃希菌生物膜形成的影响。方法:在乳胶、纯硅胶、硅橡胶材质导尿管膜片上制备大肠埃希菌生物膜,扫描电镜观察生物膜的形态。用冲洗、冲洗+超声振荡方法处理附有不同生长时期生物膜的导尿管,通过菌落计数法了解两种方法对不同时期生物膜的清除效应。结果:乳胶、纯硅胶、硅橡胶导尿管生物膜内均有细菌团块样聚集,可见纤维素样物质交联。硅橡胶导尿管生物膜内纤维素样交联物相对较少。在生物膜形成的初期,两种处理方法具有相同清除效应;但当生物膜趋于成熟后,单用冲洗方法难以将黏附细菌洗脱。结论:建议选择使用经济、实用的硅橡胶导尿管。在生物膜形成初期,对导尿管一般冲洗能较彻底地清除黏附细菌。
Objective：To investigate the effect of different catheter materials against formation of E.coli biofilm.Method：E.coli biofilms were prepared with three kinds of catheter,which were made of latex,pure silicone and silicone rubber,incubated with E.coli,and the biofilm morphology was observed by scanning electron microscope.In addition,two ways including washing,washing and ultrasound were employed to treat E.coli biofilms in different growth period.By colony counting method the two methods of biofilm removal effect in different periods were understood.Result：On the three catheter material surface there were coated with matrix by scanning electron microscopy,a great deal of bacteria adhered the fibrin matrix and clusters of bacteria cell were seen.Silicone rubber catheter within the biofilm fibrinoid cross-linked complexes was relatively less.The eradicative efficacy of various treatment showed,that two ways had similar efficacy of eradicate the young E.coli biofilms,but washing was difficult to eradicated the mature biofilm from catheter,compared with the strongest eradication of wash plus ultrasound.Conclusion：Catheters made of latex and pure silicone are easily adhered to bacteria and formation of E.coli,compared with silicone rubber;washing is effective methods to eradicate young biofilm adhered to catheters.
出处
《中国医学创新》
CAS
2014年第6期16-18,共3页
Medical Innovation of China
基金
2012年辽宁省教育厅大学生创新课题(230700409-9400880901)
关键词
导尿管
大肠埃希菌
生物膜
扫描电镜
Different catheter materials
E.coli
Biofilm
Scanning electron microscope（SEM）
