摘要
目的探讨青藤碱和柳氮磺吡啶（SASP）对小鼠实验性结肠炎的作用及其机制。方法70只SPF级雄性昆明小鼠均分为7组，除对照组（不造模、不干预）外，使用恶唑酮将所有小鼠制备成实验性结肠炎模型。造模后，每日分别予模型组、低剂量青藤碱组、高剂量青藤碱组、低剂量联合用药组、高剂量联合用药组、SASP组0．9％氯化钠溶液1mL、青藤碱（40mg／kg）、青藤碱（120mg／kg）、青藤碱（40mg／kg）＋SASP（400mg／kg）、青藤碱（120mg／kg）＋SASP（400mg／kg）、SASP（400mg／kg）灌胃1次，连续7d。记录小鼠粪便性状、体质量变化和粪隐血情况并行疾病活动指数（DAI）评分。干预结束后次日处死所有小鼠并留取结肠，行大体损伤评分。于结肠炎性反应或溃疡部位取标本用于组织学检查和损伤评分。用RT-PCR法检测结肠组织丝裂原活化蛋白激酶激酶（MKK）5、ERK5、MKK7、Jun氨基末端激酶（JNK）、NF-kB mRNA的表达水平。组间比较采用t检验。结果低剂量青藤碱组、高剂量青藤碱组、低剂量联合用药组、高剂量联合用药组、SASP组的DAl分别为2．33±0．77、1．03±0．73、2．70±0．67、1．60±0．66、2．03±0．79，其组织学损伤评分分别为5．504±1．43、4．00士1．49、6．80v1．75、4．80±1．32、5．40±1．58，均低于模型组（3．40±0．66和11．40±1．71），差异均有统计学意义（tDAl=3．33、7．61、2．34、6．08、4．18，t组织学损伤评分=8．35、10．31、5．94、9．66、8．15，P均〈0．05）。高剂量青藤碱组、高剂量联合用药组、SASP组大体损伤评分（分别为1．40±1．26、1．70±1．06、1．80±1．32）均低于模型组（3．00±1．05），差异均有统计学意义（t=3．07、2．75、2．25，P均〈O．05）。在MKK5、ERK5、MKK7、JNK、NF-KB的mRNA表达水平方面，SASP组（分别为24．29±3．40、34．74±3．05、21．34±3．74、18．71�
To investigate the role and mechanism of sinomenine and sulfasalazine（SASP） in experimental colitis of mice. Methods Seventy SPF grade Kunming mice were evenly divided into seven groups. Except control group, all mice were treated with oxazolone to create experimental colitis model. After modeling, the model group, low dose sinomenine group, high dose sinomenine group, low dose sinomenine combined group, high dose sinomenine combined group and SASP group was gavaged once daily with 0. 9% NaCI solution 1 mL, 40 mg/kg sinomenine, 120 mg/kg sinomenine, 40 mg/kg sinomenine and 400 mg/kg SASP, 120 mg/kg sinomenine and 400 mg/kg SASP and 400 mg/kg SASP alone for seven days. The mouse stool characters, changes in body weight and fecal occult blood were recorded and disease activity index （DAI） was scored. The next day after the end of the intervention, all mice were sacrificed and specimens of the colon were obtained and injury was scored. The specimens of inflammation part and ulcer site of colon were taken for histological examination and injury scoring. The expression of mitogen-activated protein kinase kinase 5 （MKK5）, extracellular signal-regulated kinase 5 （ERK5）, mitogen-aetivated protein kinase kinase 7 （MKKT）, Jun N-terminal kinase （]NK）, nuclear factor eB （NF-~cB） at mRNA level were detected by reverse transcription-polymerase chain reaction （RT- PCR）. The t-test was performed for comparison between groups. Results The DAI of low dose sinomenine group, high. dose sinomenine group, low dose sinomenine combined group, high dose sinomenine combined group and SASP group was 2.33±0.77,1.03±0.73,2.70±0.67,1.60±0.66 and 2.03±0.79, respectively, the score of injury was 5.50±1.43,4. 00±1.49,6. 80v1.75,4. 80±1.32 and 5. 40±1.58, respectively, all were lower than those of model group （3.40±0.66 and 11. 40±1.71） and the differences were statistically significant （tmai =3. 33, 7. 61, 2. 34, 6. 08 and 4. 18,t score of injury =8. 35, 10. 31, 5.94, 9.66 and 8. 15 ; all P〈0.05
出处
《中华消化杂志》
CAS
CSCD
北大核心
2014年第3期170-174,共5页
Chinese Journal of Digestion
