摘要
The diseased samples collected from Guangdong Province were inoculated to Marc-145 cells, PRRSV-specific cytopathogenic effects （CPE） such as cell aggregation, cell shrinkage and cell exfoliation were observed and a PRRSV strain XH-GD was obtained. By using primers designed in accordance with the pub- lished gene se^lences of PRRSV in GenBank, NSP2, ORF3 and ORF5 fragments were amplified by reverse transcription polymerase chain reaction and analyzed by sequence aligment. The results demonstrated that PRRSV strain XH-GD belonged to the North American genotype and the NSP2 gene contained discontinuous de- letions of 30 amino acids. NSP2, ORF3 and ORF5 genes of XH-GD shared 83.3% -98.9%, 88.6% -99.2% and 88.1% -99.2% nucleotide homology, and 76.8% -98.3%, 83.7% -98.8% and 88.1% -99.2% amino acid homology with HUB1, NX06, BJsy06, VR-2332, HB-1（sh）2002, HB-2（sh）2002, CH-1a and RespPRRS MLV, respectively; however, NSP2, ORb3 and ORF5 genes of XH-GD shared only 52.9%, 65.0% and 64. 3% nucleotide homology, and 31.3%, 57.5% and 58.9% amino acid homology with LV, respectively. Phylogenetic analysis revealed that XH-GD had a closer relationship with HUB1, NX06, BJsy06, VR-2332, HB-1 （sh） 2002, HB-2 （sh） 2002, CH-la and RespPRRS MLV strains compared with LV strain.
The diseased samples collected from Guangdong Province were inoculated to Marc-145 cells, PRRSV-specific cytopathogenic effects （CPE） such as cell aggregation, cell shrinkage and cell exfoliation were observed and a PRRSV strain XH-GD was obtained. By using primers designed in accordance with the pub- lished gene se^lences of PRRSV in GenBank, NSP2, ORF3 and ORF5 fragments were amplified by reverse transcription polymerase chain reaction and analyzed by sequence aligment. The results demonstrated that PRRSV strain XH-GD belonged to the North American genotype and the NSP2 gene contained discontinuous de- letions of 30 amino acids. NSP2, ORF3 and ORF5 genes of XH-GD shared 83.3% -98.9%, 88.6% -99.2% and 88.1% -99.2% nucleotide homology, and 76.8% -98.3%, 83.7% -98.8% and 88.1% -99.2% amino acid homology with HUB1, NX06, BJsy06, VR-2332, HB-1（sh）2002, HB-2（sh）2002, CH-1a and RespPRRS MLV, respectively; however, NSP2, ORb3 and ORF5 genes of XH-GD shared only 52.9%, 65.0% and 64. 3% nucleotide homology, and 31.3%, 57.5% and 58.9% amino acid homology with LV, respectively. Phylogenetic analysis revealed that XH-GD had a closer relationship with HUB1, NX06, BJsy06, VR-2332, HB-1 （sh） 2002, HB-2 （sh） 2002, CH-la and RespPRRS MLV strains compared with LV strain.
基金
Supported by National Natural Science Foundation of China(31272564)
Joint Fund of NSFC-Guangdong of China(U0931003)
China Agriculture Research System(CARS-36)
Hainan Provincial Research Foundation for public institutions(Qiong Cai Yu[2013]No.131)
