摘要
目的基于"肺主通调水道"理论观察中药复方泽漆化痰方对烟熏合脂多糖(LPS)气道内滴入诱导的慢性阻塞性肺疾病(COPD)大鼠模型气道黏液高分泌的影响。方法 48只大鼠随机分为空白组、模型组、阳性药物沐舒坦对照组、泽漆化痰低剂量组、泽漆化痰中剂量组、泽漆化痰高剂量组,每组8只。于第30日处死大鼠,采集标本。采用HE染色和阿辛兰-过碘酸雪夫染色(AB-PAS)的组织学检查;免疫组化检测气管和肺内气道的MUC5AC、AQP5。结果与正常组比较,模型组大鼠气管黏膜上皮黏蛋白、MUC5AC蛋白表达升高,AQP5蛋白表达降低(P<0.05)。与模型组比较,泽漆化痰高剂量组黏蛋白、MUC5AC蛋白水平降低,AQP5蛋白水平升高(P<0.05)。与沐舒坦对照组比较,泽漆化痰高剂量组MUC5AC蛋白水平降低(P<0.05)。各剂量组对模型大鼠肺内气道的总黏蛋白、MUC5AC、AQP5水平的影响不明显。结论中药复方泽漆化痰方较高剂量具有抑制气道黏液高分泌的作用,且影响部位以大气道为主。
Objective：To study the influence of Zeqihuatan Decoction on airway mucus hypersecretion in rats based on the theory of lung governing regulation of water passages.Methods：48 wistar rats were randomly divided into blank group,model group,control group,asthmatic group,Zeqihuatan Decoction low dose group,Zeqihuatan Decoction middle dose group and Zeqihuatan Decoction high dose group,with 8 rats for each group.In thirtieth day the rats were killed to be collected as specimens.Histological examinations were performed by HE staining and AB-PAS staining; Protein MUC5AC and AQP5 were measured by immunohistochemistry.Results：Compared with normal group,tracheal epithelial mucin and MUCSAC protein expression increased and AQP5 protein expression decreased in model group（P＜ 0.05）.Compared with model group,MUC5AC protein levels decreased and AQP5 protein levels increased in Zeqihuatan Decoction high dose group （P＜0.05）.Compared with control group,MUC5AC protein levels in Zeqihuatan Decoction high dose group decreased （P＜ 0.05）.There was no statistical significance among pulmonary airways of different groups on total mucin,MUCSAC and AQP5 protein levels.Conclusion：This study shows that higher dose Zeqihuatan Decoction of Chinese herbal compound can inhibit airway mucus hypersecretion with large airway being the main point of action.
出处
《中国中医急症》
2014年第3期391-393,共3页
Journal of Emergency in Traditional Chinese Medicine
基金
国家中医药管理局黄吉赓名老中医药专家传承工作室(704)
