摘要
洋桔梗是国际上十分流行的盆花和切花种类。以洋桔梗‘圣剑’无菌苗叶片为外植体,研究了6-BA与NAA不同浓度组合对其不定芽再生的影响,并分别比较了不同浓度IBA和NAA诱导其生根的效果,测定了该品种在不定芽再生时对卡那霉素(Km)的敏感性。结果表明:MS+0.5 mg·L-16-BA+0.01 mg·L-1NAA为不定芽再生最适培养基,不定芽再生率达91%;1/2 MS+0.2 mg·L-1IBA为不定根再生的最适培养基,生根率达89%;抑制叶片不定芽再生的Km最低浓度为25 mg·L-1。建立了‘圣剑’洋桔梗植株高频再生体系,并确定了其对卡那霉素的敏感性,为该品种的基因工程研究奠定了基础。
Eustoma grandiflorum is a popular cut flower and pot flower in the world. In this study, using leaves of sterile plantlets of E. grandiflorum cv. Excalibur Blue Picotee as explants, the effects of different concentration com- binations of 6-BA and NAA on adventitious bud regeneration were investigated. The effects of different concentra tions of IBA and NAA on rooting were studied, respectively. The sensitivity to kanamycin upon the regeneration of adventitious buds was determined. The results showed that the medium with MS＋0.5 mg. L-1 6-BA＋0.01 mg.L-1 NAA was the optimum medium for adventitious bud regeneration, with 91% regeneration rate. Meanwhile, the medi- um with 1/2MSq-0.2 mg.L-1 1BA was the optimum medium for rooting,and the rooting rate reached 89 %. Besides, the minimum concentration of Km inhibiting the regeneration of adventitious buds was 25 mg . L-1. This study laid an important foundation for the research of genetic engineering on this cultivar of E. grandiflorurn.
出处
《广西植物》
CAS
CSCD
北大核心
2014年第1期120-125,共6页
Guihaia
基金
江苏省高校优势学科建设工程项目
关键词
洋桔梗'圣剑’
植株高频再生体系
卡那霉素敏感性
Eustoma grandiagorum ＇Excalibur Blue Picotee＇
high frequency plant regeneration system
kanamycinsensitivity
