摘要
以番茄品种Alpatieva 90 5a和品系Line 1的子叶、下胚轴为外植体诱导愈伤组织进行细胞育种 ,培养基中附加不同浓度的制霉菌素 (Nystatin)用于突变体的筛选 ,结果表明 :两个品种制霉菌素的临界致死浓度 (仅存活 2 %微型愈伤组织时的浓度 )分别为 14 0mg/L和180mg/L。再生植株分别在培养皿内 (试管苗 )和温室等条件下进行了抗晚疫病鉴定 。
Employing both cotyledon and hypocotyl of Russia tomato cultivar of Alpatieva 905a and its line of Line 1 as the explants of the experimental materials,the cellular breeding for resistance to Phytothphora infestans( Mont.)De Bary〕was conducted through tissue culture by adding various concentrations of Nystatin a antibiotic chemical in MS medium.The result showed that the median lethal doses of Nystatin(the concentration at that 2% of the micro calli survived)were 140mg/L or 180mg/L for both Alpatieva 905a and Line 1,respectively.Retest results in the test tube or at greenhouse also showed that the regenerated seedlings had the higher resistances to Phytothphora infestans than the control.
出处
《园艺学报》
CAS
CSCD
北大核心
2000年第5期377-379,共3页
Acta Horticulturae Sinica
基金
教育部留学回国人员科研启动费资助项目
关键词
晚疫病
番茄
突变体
制霉菌素
抗病性
Phytophthora infestans 〔(Mont.)De Bary〕
Tomato
Mumant seedlings
Antibiotic nystatin
