摘要
目的:建立测定加味水陆丸中金丝桃苷含量的方法。方法:采用DIONEX-C18柱(4.6 mm×250 mm,5μm)为色谱柱,以乙腈:0.1%磷酸溶液(10∶90)为流动相,流速:1.0 mL·min-1,柱温:30℃,检测波长:360 nm。结果:金丝桃苷在4.0-80.0μg/mL浓度范围内有良好线性关系(r=0.9998),平均加样回收率为97.6%,RSD%=1.26%。结论:本方法简便,快速,准确,重复性好,可用于加味水陆丸中金丝桃苷的含量测定。
Objective： To establish a HPLC method for the determination of Hyperoside in Jiawei Shuilu wan. Methods： The HPLC system consisted of the DIONEX-C18 （4.6 mm×250 mm, 5 μm）column, the mobile phase consisted of acetonitrile（10） - 0.1% H2PO4 （90）; the flow rate was 1.0 mL＇min-I; the column temperature was 30℃; The UV detector was set at 360 nm. Results： The linear response range was 4.0-80.0 μg/mL（r=0.9998）. The average recovery of hyperoside was 97.6%, and RSD% =1.26%. Conclusions： The method is simple, rapid, accurate and repeatable. It can be applied in determination of Hyperoside in Jiawei Shuilu wan.
出处
《中医药导报》
2014年第3期72-73,共2页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
