摘要
背景:前期实验构建的羟基丁酸-羟基辛酸共聚体一体化骨软骨支架具备良好的生物相容性、生物可降解性,并且降解产物无毒性。目的:将兔肾微血管内皮细胞与羟基丁酸-羟基辛酸一体化骨软骨支架复合培养,观察支架骨层血管化效果。方法:运用溶剂浇铸-颗粒沥滤法,制备具有骨层/骨与软骨界面层/软骨层3层结构的羟基丁酸-羟基辛酸一体化骨软骨支架。将传代培养至第3代的兔肾微血管内皮细胞,接种到一体化骨软骨支架骨层支架上,MTT法检测细胞在支架上的增殖活性,10 d后苏木精-伊红染色及电镜观察细胞在支架内的生长状况。结果与结论:一体化骨软骨支架外观具备明显的3层结构,各层之间连接紧密,骨层疏松多孔,各层支架孔隙均匀且相通,一体化支架孔隙率为78%。兔肾微血管内皮细胞在支架上分裂增殖良好,复合培养10 d后,细胞在骨层支架内呈立体生长,中间界面层内未发现细胞,苏木精-伊红染色可见细胞黏附生长于骨层支架孔隙间,细胞依附支架的多孔结构生长,形成管腔样结构,但细胞并未长入中间界面层。
BACKGROUND：The poly（hydroxybutyrate-co-hydroxyoctanoate） osteochondral scaffold which has been constructed in previous experiments has good biocompatibility and biodegradability and generates non-toxic degradation products. OBJECTIVE：To observe the vascularization of rabbit renal microvascular endothelial cels co-cultured with poly（hydroxybutyrate-co-hydroxyoctanoate） osteochondral scaffold. METHODS：The poly（hydroxybutyrate-co-hydroxyoctanoate） osteochondral scaffold having a three-layer structure （layer of bone/bone and cartilage interface layer/layer of cartilage） was prepared by solvent casting/particle leaching method. The renal microvascular endothelial cels at passage 3 were seeded onto the scaffold of bone layer. The proliferation of the renal microvascular endothelial cels growing on the scaffolds was examined using 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide method, the growth of cels in the scaffold was observed by hematoxylin-eosin staining under electron microscope after 10 days. RESULTS AND CONCLUSION：The integrated osteochondral scaffold had a clear appearance of three-layer structure, which had closed connections between the three layers. Porous bone layer was visible as wel as uniform and interlinked pores, and the porosity was 78%. The renal microvascular endothelial cels seeded onto＆nbsp;the scaffold proliferated wel and presented a three-dimensional growth after 10 days of co-culture, but there were no cels on the interface layer. Cels which adhered and grew between the pores of the bone layer were observed through hematoxylin-eosin staining. Cels showed a luminal-like structure growing on the scaffold with the porous structure, but they did not grow into the interface layer of bone and cartilage.
出处
《中国组织工程研究》
CAS
CSCD
2014年第8期1185-1190,共6页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金(主任基金)项目(31040030)
项目名称:新型多聚羟基烷酸软骨组织工程载体的研究~~
