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实时荧光定量PCR法检测mRNA与免疫方法检测蛋白的对比分析

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摘要 目的比较不同检测技术对结肠癌患者外周血中细胞角蛋白19(CK19)基因的表达。方法纳入50例合格患者,50例健康者为对照组。采用实时荧光定量PCR法检测mRNA与免疫方法检测蛋白。结果实时荧光定量PCR法阳性率为60%。其中对低分化类型阳性率为80%。Duke's分期的A型、D型为100%。无局部、远处转移患者阳性率分别是63.16%、72.73%。免疫方法有40例为阳性,阳性率为80%。其中对有局部、远处转移患者阳性率分别为96.77%、100.0%。结论免疫方法检测阳性率较高,但是实时荧光定量PCR法检测更为灵敏,对早期、低分化类型更有优势。
出处 《中国医学工程》 2014年第1期49-50,共2页 China Medical Engineering
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