摘要
目的 :研究胃肠道肿瘤患者外周血、引流静脉血中CEAmRNA、CK2 0mRNA的表达与胃肠道肿瘤的临床病理联系及预后的关系。方法 :以CEAmRNA、CK2 0mRNA为指标 ,用RT PCR方法检测对照组 10例外周血 ,14例胃癌和 11例结直肠癌外周血及腹腔内引流静脉血中的肿瘤细胞。 结果 :对照组 10例无CEAmRNA、CK2 0mRNA表达 ,CEAmRNA、CK2 0mRNA在 2 5例胃肠道肿瘤患者外周血、引流静脉血中的阳性表达率分别为5 / 2 1(2 3.8% )、12 / 2 4 (5 0 % ) ,0 / 2 1(0 % )、0 / 2 4 (0 % )。CEAmRNA阳性表达率高于CK2 0mRNA阳性表达率 (P <0 .0 5 )。CEAmRNA在引流静脉血中的阳性率 (5 0 % )高于外周血中阳性率 (2 3.8% ) ,差异有统计学意义 (P <0 .0 5 )。 3例外周血、引流静脉血CEAmRNA阳性的患者半年内出现复发和转移。 2 5例病例中未发现CEAmRNA阳性表达与年龄、性别、肿瘤的浸润深度、分化、分期及淋巴结转移相关。 结论 :CK2 0mRNA在外周血、引流静脉血中表达非常不稳定 ,以CEAmRNA、CK2 0mRNA为指标监测肿瘤的复发或者作为预后的指标还需要扩大样本进一步的研究。
Objective: To study the expression of CEA mRNA, CK20 mRN A in the peripheral and leading venous blood of patients with gastrointestinal carcinoma, and to explore its clinical significance. Methods: A total of 25 patients preoperative peripheral venous blood and mid-operative leading venous blood specimens were obtained. RT-PCR assay was applied to detect the expression of CEA mRNA, CK20 mRNA. As control, 10 patients were obtained,which were free from malignent gastrointestinal diseases. Results: The positivity for CEA mRNA and CK20 mRNA expression in the peripheral and leading venous blood with gastrointestinal carcinoma patients were 5/21(23.8%)、12/24(50%),0/21(0%)、0/24(0%) respectively, but none in those controls. CEA mRNA tended to be more sensitive than CK20 mRNA as a marker. The patients with all positive for CEA mRNA in the peripheral and leading venous blood appeared recurrence during half year. The CEA mRNA expression was not correlated with sex, age, differentiation, clinical pathologic stage, lymph metastasis and depth of invasion. Conclusion: CEA mRNA, CK20 mRNA as a marker for detecting subclinical metastasis, recurrence, assessing the prognosis needed to be further studied.
出处
《新疆医科大学学报》
CAS
2004年第6期597-599,共3页
Journal of Xinjiang Medical University
