摘要
目的探讨黄芪桂枝五物汤中总黄酮对小鼠免疫功能的影响,从而为临床应用提供参考。方法取小鼠脾淋巴细胞,加入不同浓度的总黄酮,采用水溶性四唑盐(WST-1)法测定细胞的增殖活性;酶联免疫吸附试验(ELISA)测细胞因子白细胞介素-2(IL-2)和干扰素γ(IFN-γ)含量;流式细胞仪检测小鼠脾细胞中CD3+、CD19+细胞。结果各浓度总黄酮组淋巴细胞增殖活性、IL-2、IFN-γ及CD3+细胞显著高于对照组(P<0.05,P<0.01))。结论总黄酮可增强免疫功能,但只能提高细胞免疫功能,对体液免疫功能无明显影响。
Objective To research the effect of total flavones extracted from Huangqi guizhi wuwu decoction on immune function mice on in vitro ,and to provide a reference for the clinical application .Methods Splenic lym-phocyte of mice were extracted ,and stimulated with different concentration flavones .Proliferations of the cells were de-tected with WST-1 assay;IL-2,IFN-γsecreted by the cells were examined with enzyme linked immune sorbent assay （ ELISA）;Cell subtypes were checked with flow cytometry （ FCM ） .Results Compared with the control group,proliferation of the cells and concentration of IL -2,IFN-γand CD3 ＋in different concentration total flavones group increased significantly （P＆lt;0.05,P＆lt;0.01）.Conclusion The total flavones might enhance cellular immune function but not humoral immune function .
出处
《河北中医》
2014年第3期432-434,共3页
Hebei Journal of Traditional Chinese Medicine
基金
2013年唐山市科学技术研究与发展计划增补项目(编号:13130253z)
