摘要
目的：探讨冷束缚应激大鼠血浆及胃粘膜中β－内啡肽含量的变化及作用。方法：制作大鼠冷束缚应激模型，以放射免疫法测定应激０ｍｉｎ（对照组）、１５ ｍｉｎ、２ｈ及８ｈ时大鼠血浆及胃粘膜中β－内啡肽的含量，同时测定胃液ｐＨ和胃粘膜溃疡指数（ＵＩ）。另设两组预处理组，分别于应激前１５ ｍｉｎ经颈静脉注射纳洛酮（１．６ ｍｇ／ｋｇ）或等容量生理盐水（０．８ｍｇ／ｋｇ），测定其应激２ｈ时的胃液ｐＨ及胃粘膜ＵＩ。结果：整个应激过程中，大鼠血浆及胃粘膜中β－内啡肽含量无显著变化（Ｐ＞０．０５）；应激各组大鼠的胃粘膜ＵＩ均显著高于对照组（Ｐ＜０.０１）；应激１５ ｍｉｎ组大鼠的胃液ｐＨ显著高于对照组（Ｐ＜０．０１），临终前（应激８ｈ组）大鼠的胃液ｐＨ亦显著高于对照组（Ｐ＜０．０５）；纳洛酮组大鼠的胃液ｐＨ及胃粘膜ＵＩ较生理盐水对照组无显著差异（Ｐ＞０．０５）。结论：应激过程中大鼠血浆及胃粘膜中β－内啡肽含量稳定，阻断β－内啡肽受体不影响胃液ｐＨ及胃粘膜ＵＩ；β－内啡肽对应激所致的胃粘膜损伤可能不起重要作用。
Background/Aims: To evaluate the role and changes of β-endorphin in plasma and gastric mu- cosa of rats after cold restraint stress. Methods: 60 rats were used in this study. The cold restraint stress model of rat was made. At 0 min (control group), 15 min, 2h and 8 h after the stress, the levels of β-endorphin in plasma and gastric mucosa were assessed with radioimmunoassay. Gastric juice pH,gas- tric ulcer index (UI) were also measured. Gastric juice pH and gastric UI were measured at 2 h after stress in the groups pretreated intravenously with Naloxone (1.6 mg/kg) or saline (0.8 mg/kg). Results: The levels of β-endorphin in plasma and gastric mucosa during the stress process were not changed (P>0.05). Gastric UI of all stress groups rose significantly as compared with the control group (P< 0.01). Gastric juice pH at 15 min and 8h stress process were significantly higher than that of the con- trol group (P<0.01 and P<0.05). Pretreatment group with Naloxone did not show any change in gas- tric juice pH and gastric UI (P>0.05). Conclusions: β-endorphin levels in plasma and gastric mucosa in all stress process are stable, pretreatment with Naloxone has no effects on gastric juice pH and gas- tric UI. β-endorphin may not play an important role in gastric stress damage.
出处
《胃肠病学》
2000年第4期229-230,239,共3页
Chinese Journal of Gastroenterology
基金
1997年全军杰出中青年医药卫生基金
