摘要
通过紫外可见光光谱、荧光光谱、同步荧光光谱、瑞利散射共振光谱等技术研究发现,活性艳橙K-7R(RK-7R)与丝素肽结合为1∶1、作用距离为3.46 nm的非荧光性复合物。活性艳橙K-7R印染体系中存在的阳离子表面活性剂CTAB、3种离子液体(溴化1-乙基-2,3-二甲基咪唑、1-乙基-2,3-甲基咪唑双三氟甲磺酰亚胺盐、氯化羟乙基三甲基胺)、3种金属离子(Ca2+、Pb2+、Ba2+)都增强了丝素肽与活性艳橙K-7R的结合;而非离子型表面活性剂曲拉通X-100以及Cu2+均削弱二者结合,阴离子表面活性剂十二烷基磺酸钠对二者结合无影响。
To investigate the interaction between silk peptide and Reactive Orange K-7R （RK-7R）, multiple spectroscopy technologies. （ ultraviolet visible spectroscopy, fluorescence spectroscopy, synchronous fluorescence spectroscopy and Rayleigh resonance） have been used. The results showed that a non fluorescence complex was formed between the silk peptide and K-7R. The binding distance and binding ratio were calculated as 3.46 nm and 1 ： 1, respectively. The binding constants increased when ionic liquids including chloride hydroxyethyl trimethyl amine, bromide 1-ethyl-2,3-dimethyl imidazolium, 1-ethyl-2,3-methyl imidazolium bis-[ trifluoromethyl-sulfonyl ] imide, metal ions （ Ca2＋ , Pb2＋ and Ba2＋）, and suffactants CTAB were added into the SP/ K-7R system, while decreased when Cu2＋ and Triton X-100 were in the presence. Sodium dodecyl sulfate had no effects on the system.
出处
《纺织学报》
EI
CAS
CSCD
北大核心
2014年第3期81-86,共6页
Journal of Textile Research
基金
陕西省科学计划项目(2013KJ07-24)
西安工程大学自然科学基金资助项目(09XG02)
西安工程大学博士启动基金资助项目(09BS018)
关键词
丝素肽
分子光谱
结合作用
silk peptide
Reactive Orange K-7R
spectroscopy
interaction
