摘要
目的利用电感耦合等离子体一质谱（ICP—MS）建立工作场所空气和人体血、尿中多种金属及其化合物的同时快速检测方法。方法工作场所空气样品经消化，血样和尿样直接稀释后，采用ICP-MS进行定量分析。结果待测元素在实验选定的范围内具有良好的线性关系。空气样品中铍（Be）、钒（V）、铬（cr）、锰（Mn）、钴（co）、镍（Ni）、铜（Cu）、锌（Zn）、钼（Mo）、镉（Cd）、锑（Sb）、钡（Ba）、铊（T1）和铅（Pb）14种元素的检出限为0．02～6．50μg/L，最低检出质量浓度为2．40×10^-6—8．40×10^4mg／m^3，相对标准偏差（RSD）为0．93％～5．64％。血样中Be、V、Cr、Mn、Co、Ni、Cu、Zn、镓、锗、锆、Mo、Cd、铟、锡、Sb、碲、Ba、钨、砷、硒、TI、Pb和铋24种元素的检出限为0．02～2．88μg/L，RSD为0．74％～10．67％，标准物质的测定结果均在参考值范围内。尿样中24种元素的检出限为0．02—1．39μg/L，RSD为0．65％-9．34％。结论该法快速、准确、简便，可用于职业卫生工作场所空气、生物材料样品金属及其相关化合物的定量检测，也可用于重金属中毒事故的快速筛查。
Objective To develop a rapid and simultaneous detection method for metals and their compounds in workplace air, human blood and urine with inductively coupled plasma-mass spectrometry （ICP-MS）. Methods The digested sample of air, and the samples of blood and urine were directly detected by ICP-MS. Results Good linear relationships in the se-lected range were observed, the detection limits of 14 elements including Be, V, Cr, Mn, Co, Ni, Cu, Zn, Mo, Cd, Sb, Ba, T1 and Pb in workplace air were 0. 02-6. 50 μg/L, the minimum detectable qualitical concentrations were 2.4 × 10^ -6. 8.4 × 10^ -4 mg/m^3 （ sample volume was fixed to 75 L）, and the relative standard deviation （RSD） was 0. 93%-5.64%. The detection limits of 24 elements including Be, V, Cr, Mn, Co, Ni, Cu, Zn, Ga, Ge, Zr, Mo, Cd, In, Sn, Sb, Te, Ba, W, As, Se, T1, Pb and Bi were 0. 02-2. 88 μg/L in blood, and 0. 02-1.39 μg/L in urine respectively. RSD were 0. 74%-10. 67% in blood and 0. 65%-9.34% in urine respectively. Determination resuhs of standard substance were within reference values. Conclusion The method is rapid, accurate and simple, it can be used for quantitative detection for occupational hygiene in workplace air, and biological material sample including metals and their compounds, it also can be used for rapid screening of heavy metal poisoning.
出处
《中国职业医学》
CAS
北大核心
2014年第1期56-60,共5页
China Occupational Medicine
基金
广东省职业病防治重点实验室(2012A061400007)
关键词
电感耦合等离子体质谱
职业卫生
元素
重金属
空气
血液
尿液
检测
Inductively coupled plasma mass spectrometry
Occupational health
Elements
Heavy metal
Workplace air
Blood
Urine
Detection
