摘要
目的：观察赤雹果总有机酸（TOATDF）的解热作用,并探讨其机制.方法：SD大鼠ip内毒素（LPS） 100 μg·kg-1制备大鼠发热模型.将模型大鼠随机分为TOATDF 300,150,75 mg· kg-1剂量组,阿司匹林150 mg·kg-1对照组及模型组,另取10只大鼠作为正常对照.药后每1h测量体温1次,连续6次.采用酶联免疫测定法（ELISA）法测定血清中前列腺素E2（PGE2）、肿瘤坏死因子-α（TNF-α）和白介素（IL）-1β及脑脊液中PGE2表达水平.结果：TOATDF 300,150 mg·kg-1剂量组大鼠各时间点的平均体温均低于模型组（P ＜0.05或P＜0.01）;75 mg·kg-1组大鼠药后2 ～5h体温明显低于模型组（P＜0.05）;阿司匹林组药后1～5h体温明显低于模型组.各给药组血清中TNF-α,PGE2,IL-β及脑脊液中PGE2的表达水平均显著低于模型模型组（P＜0.05或P＜0.01）,但高于正常对照组（P＜0.05）.结论：TOATRF有明显的解热作用,并有一定的剂量-效应关系.其解热作用与抑制致热原诱发的PGE2,TNF-α和IL-β等致热因子的产生或释放有关.
Objective：To investigate the antipryretic effects and its possible mechanism of total arganic acid of Thladiantha dubia fruit （TOATDF）.Method：The rat fever model was established by injection of lipopolysaccharides （LPS） of 100 μg ·kg-1 and then the model rats were randomly divided into five groups：TOATDF 300,150,75 mg·kg-1 groups,Asprin 150 mg ·kg-1 group （positive control）,and model control group,at the same time,normal rats as the control group.All groups were ig given the same volume drugs or saline.After treatment temperature was measured once each hour for 6 times.The levels of prostaglandin E2 （PGE2）,tumor necrosis factor-α （TNF-α） and interleukin-1β （IL-1β） in serum and PGE2 in cerebrospinal fluid were measured by enzyme linked immunosorbant assay （ELISA）.Result：At each time point,the average temperature in TOATD 300,150 mg ·kg-1groups was lower than that in the model control group （P ＜0.05 orP ＜ 0.01）,in TOATDF 75 mg ·kg-1 group and asprin group,the average temperature was lower than that in the model control group 2-5 h and 1-5 h after treatment,respectively.The expression levels of PGE2,TNF-α and IL-β in serum and PGE2 in cerebrospinal fluid were significantly lower than that of model group （P ＜ 0.05 or P ＜ 0.01）,but higher than that of normal control group （P ＜ 0.05）.Conclusion：TOATDF has significant antipyretic effect on LPS-induced febris response in rats and in a dose-dependent manner.Its mechanism may be related to inhibiting expression of PGE2,TNF-α and IL-β in serum and PGE2 in cerebrospinal fluid.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第6期154-157,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河北省自然科学基金项目(C200600863)
