摘要
目的应用星点设计-响应面法优化石墨炉原子吸收测定小鼠睾丸组织中镉的实验条件。方法以混合基体改进剂用量（0.3-3．7ml）、灰化温度（252～588℃）和原子化温度（1464～2136℃）为考察对象，以吸光度值作为响应值，进行3因素5水平星点设计，建立数学模型，通过响应面法预测最佳测定条件，并对其进行验证。结果响应值与因素之间可采用二项式拟合，决定系数为0．9851。采用优化的条件（灰化温度为400℃，原子化温度为2020℃，混合基改加入量为2．4m1）测定镉，实测值与预测值仅有1．3％的差别。在此条件下，测定镉的线性范围为0．5～6．0μg／L，RSD为1．19％，检出限为0．136μg／L，平均加标回收率为98．9％。结论星点设计-响应面优化法可用于石墨炉原子法测定条件的优化，所建立的数学模型预测性良好。
Objective To employ response surface method based on central composite design to optimize deterrnination condition of graphite furnace atomic absorption spectrometry （GF-AAS） for the determination of cadmium in testis of mouse. Methods Dosage of mixed matrix modifier （0.3-3.7 ml）,ashing temperature（252-588 ℃） and atomization temperature （1 464-2 136 ℃） were selected as the influencing factors and the response value was absorbanee. The experiment mathematical model was arranged according to four-factor, five-level central composite design,and the optimal conditions were predicted by response surface method. Results Absorbance was simulated by using second-order polynomial equation and the regression coefficient was 98.51%. The results showed that the optimum extraction conditions were as follows： ashing temperature was 400 ℃, atomization temperature was 2 020 ℃ and dosage of matrix modifier was 2.4 ml. The experimental value had 1.3% difference with the predicted value under the optimum conditions. A good linearity was obtained for cadmium in the range of 0.5-6.0 μg/L with the lower limit of detection of 0.136 μg/L and the relative standard deviation （RSD） of 1.19%. The average recovery was 98.9%. Conclusion Central composite design and response surface method can be used to optimize test condition of GF-AAS and the model developed in this study has good predictability.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2014年第1期54-56,共3页
Journal of Environment and Health
基金
广东省中医药管理局项目(20122111)
关键词
镉
石墨炉原子吸收法
星点设计-响应面法
Cadmium
Graphite furnace atomic absorption spectrometry
Central composite design-response surface method
