摘要
目的探讨Runx3基因启动子区CpG岛甲基化状态和蛋白表达在锯齿状病变发生与癌变通路中的作用及意义。方法用TaqMan探针为基础的实时定量PCR(Methylight)法检测77例锯齿状病变(29例HP、29例SSA/P和19例TSA)、16例正常结直肠组织和14例结直肠癌中Runx3基因CpG岛甲基化状态,同时用免疫组化法检测相应组织中Runx3蛋白的表达;并分析两者之间的关系。结果 Runx3启动子CpG岛甲基化率在正常和HP、SSA/P、TSA、CRC分别为12.5%(2/16)和17.2%(5/29)、51.7%(15/29)、63.2%(12/19)和78.6%(11/14)。Runx3蛋白阳性表达率在正常和HP、SSA/P、TSA、CRC分别为81.3%(13/16)和72.2%(21/29)、48.3%(14/29)、31.6%(6/19)、21.4%(3/14)。SSA/P、TSA和CRC 3组中Runx3甲基化与Runx3蛋白表达结果具有相关性(P<0.05),且为负相关。结论 Runx3基因启动子区域甲基化是诱导Runx3蛋白表达下调或缺失的主要原因,在锯齿状病变尤其是锯齿状腺瘤的发生及癌变通路中起重要作用。
Objective To investigate the role and significance of methylation status and protein expression of the Runx3 gene in serrated lesions and carcinogenesis pathway. Methods The Runx3 gene promoters methylation were detected with the Taqman probe based real-time PCR（Methylight） technology in 77cases serrated lesions（29 cases of hyperplastic polyps, 29 cases of sessile serrated adenoma/polyp and 19 traditional serrated adenoma）, 16 cases of normal mucosa tissues and 14 cases of colorectal cancer. At the same time, immunohistochemical staining was u tilized to detect the expression of Runx3, and to analyze the relationship of them. Results The rates of methylation of the Runx3 gene in normal mucosa tissues, HP, SSA/P, TSA and CRC were 12.5% （2/16）, 17.2% （5/29）, 51.7 % （ 15/29 ）, 63.2 % （ 12/19 ） and 78.6 % （ 11 / 14 ）. The results of Immunohistochemistry showed that the posi- tive expression of Runx3 in normal mucosa tissues, HP, SSA/P, TSA and CRC were 81.3% （ 13/16）, 72.2% （21/29）, 48.3% （14/29）, 31.6% （6/19）and 21.4% （3/14）. The Runx3 methylayion was negatively correla ted with the expression of Runx3 protein in SSA/P, TSA and CRC （P 〈 0. 05）. Conclusions Runx3 gene promot er region methylation explains the down-r6gulation of Runx3 protein expression, which may function in the mainreasons for the serrated lesions and occurrence of serrated adenoma and cancer.
出处
《基础医学与临床》
CSCD
北大核心
2014年第3期339-344,共6页
Basic and Clinical Medicine
基金
首都卫生发展科研专项基金(2011-5021-02)
