摘要
目的探讨miR-146a对肿瘤相关巨噬细胞(TAM)功能的影响及其在TAM中表达水平变化的调控机制。方法分别对比BALB/c小鼠4T1移植瘤TAM和腹腔巨噬细胞(PEC)、人乳腺癌组织TAM和外周血单核细胞,q-PCR法检测miR-146a的表达变化。转染miR-146a antagomir的巨噬细胞与4T1混合接种,确定TAM中miR-146a表达对肿瘤发生发展的影响。结果 miR-146a在荷瘤小鼠(P<0.05)和人乳腺癌(P<0.01)相关巨噬细胞中显著下调。miR-146a inhibitor明显抑制肿瘤的生长。结论 TAM中miR-146a可能通过其对巨噬细胞的调控进而促进肿瘤生长。
Objective To investigate the influence of miR-146a on the function of tumor-associated macrophage （TAM）, and the mechanism of its expression regulation. Methods We compared TAM in 4T1 xenografted tumor grown in BALB/c mouse to peritoneal macrophage （PEC） , and TAM in human breast cancer to PBMC respectively to detect the expression of miR-146a using q-PCR method. Mixed subcutaneously implantation of miR-146a an tagomiR-transfected macrophages with 4T1 ceils was used to determine the function of miR-146a in TAM. Results miR-146a was significantly down-regulated in breast cancer-associated macrophage in both 4T1 tumor grown in mice （ P 〈 0. 05 ） and breast cancer patients （ P 〈 0. 01 ）. It was observed that macrophage with decreased miR- 146a expression inhibited the 4T1 tumor cell growth in vivo. Conclusions miR-146a can regulate the function of tumor associated macrophage, so as to promote tumor growth.
出处
《基础医学与临床》
CSCD
北大核心
2014年第3期310-313,共4页
Basic and Clinical Medicine
基金
国家自然科学基金(81171980
91029735)
