摘要
目的通过多时间点活体磁共振成像及组织取材病理检查,探讨钳夹法建立大鼠视神经损伤模型的可靠性及核磁共振成像在视神经损伤模型中的应用价值。方法健康SD大鼠36只,随机分为对照组(6只)和实验组(30只)。使用夹持力50g夹持钳持续钳夹10 s的方法建立大鼠单侧视神经损伤模型,在损伤后3 d、5 d、7 d、14 d、2个月分别对受损侧视神经进行磁共振成像扫描,并随后取视网膜及视神经进行病理检测。结果磁共振成像检查结果显示,对照组大鼠双侧视神经粗细均匀,周围无明显病变信号。损伤后3 d,实验组损伤侧可出现视神经肿胀、增粗,伴随眼球后方软组织水肿,并在随后5～14 d内呈进行性加重;至损伤后2个月,可观察到损伤侧视神经球后段萎缩。HE染色检查结果显示,实验组损伤后3～7 d,视神经出现大量空泡变性,神经纤维稀疏,胶质细胞增生明显,视网膜神经纤维层水肿明显,神经节细胞层出现核固缩和空泡;损伤后14 d,梗死灶内胶质细胞增生,纤维组织相对增多,神经纤维层结构仍显疏松;损伤后2个月,可见神经纤维增生,胶质细胞数目明显减少。对照组大鼠视神经纤维排列密集、规则,视网膜层次清晰,神经纤维层厚度均匀。结论固定压力钳夹法能建立稳定的大鼠视神经损伤模型,可获取随时间变化的视神经及视网膜细胞病理改变图像。同时,磁共振成像能显示创伤后视神经水肿及后期萎缩的变化过程,具有一定的研究价值和临床应用潜力。
Objective To investigate the reliability of establishing optic nerve clamping injury model and the value of magnetic resonance imaging （MRI） through the multi-time MRI in vivo and histopathology. Methods A total of 35 healthy SD rats were randomly divided into control group（6 cases） and experimental group（30 cases）, the fLxed 50 g pressure micro vascular clamp clamps with 10 seconds was used to estab- lish rat unilateral optic nerve（ON） injury model. MRI studies were performed at 3 days, 5 days,7 days, 14 days and 60 days after ON crushing, then the changes in morphology of the retina and ON were observed by HE staining. Results MRI studies found that the dual optic nerve were normal with no obvious pathologic changes, the thicken and swollen optic nerve and edema in surrounding tissues could be observed at 3 days in ex- perimental group with progressive increasing from 5 days to 14 days. However, shrinking nerve was obviously thinner than the controlling eyes at 60 days in experimental group. HE staining showed that from 3 days to 7 days after crushing, a large number of vacu- oles degeneration appeared in ON with scattering nerve fiber and glial cell proliferation. The edema of retinal nerve fiber layer was significant, the retinal ganglion cell layer had nuclear pyknosis and cavitation;At 14 days after injury,the proliferation of glial cell in infarcts were observed, fibrous tissue increased and retinal nerve fiber layer scattered; At 2 months after injury,the nerve fibers was proliferated and glial cells decreased sig- nificantly. In control group, the optic nerve fiber regularly arranged, the retinal layers were clear, and the retinal nerve fiber layer thickness was uniform. Conclusion Fixed pressure clamping method can es- tablish stable optic nerve injury model of rats and the pathological changes of optic nerve and retinal cell over time are ob- served. MRI can show the changing process of post-traumatic nerve edema and atrophy with certain research value and po- tential clinical applicati
出处
《眼科新进展》
CAS
北大核心
2014年第3期205-208,共4页
Recent Advances in Ophthalmology
基金
国家自然科学基金资助(编号:31160206)~~
关键词
大鼠
视神经夹持伤模型
磁共振成像
病理过程
rat
optic nerve clamping injury model
magnetic resonance imaging
pathologic processes
