摘要
目的：研究血链球菌细菌素对牙龈卟啉单胞菌（Porphyromonas gingivalis P．g）生长曲线的影响。探讨血链球菌细菌素对牙龈卟啉单胞菌细胞内钙离子浓度的影响。方法：通过低温高速离心，超声破碎等方法提取血链素，使血链素作用P．g，于37℃厌氧培养：采用微量液体对倍稀释法测定血链素对P．g的最小抑菌浓度（MIC）。选用不同浓度的血链素作用P．g，分别于0、4、8、12、16、20、24、48h时测定各组细菌悬液在590nm波长处的A值，并绘制生长曲线。用Fluo-3／AM负载P．g细胞内钙离子，在激光共聚焦显微镜下观察Ca抖荧光强度变化。结果：血链素对P．g有显著抑制作用，其MIC为0．0625g／L。血链素作用后，P．g的生长曲线在8～16h发生显著改变，对数生长期被延迟了8h。血链素作用后，P．g细胞内钙离子浓度显著降低，与对照组相比差异具有统计学意义（P〈0．01）。结论：血链球菌细菌素对牙龈卟啉单胞菌有明显的抑制作用，在本实验范围内，随着血链素浓度的升高其抑制作用逐渐增强。血链球菌细菌素可导致牙龈卟啉单胞菌细胞膜钙离子通透性增强。
Objective： To study the effects of Streptococcus sanguis bacteriocins on the growth curve the intracellular free calcium concentration of Porphyromonas gingivalis. Methods.. We extracted Streptococcus sanguis bacterio- cins by low temperature high speed centrifugal and ultrasonic broken, made Streptococcus sanguis bacteriocins affect P. g at 37℃ anaerobic culture. Trace liquid times dilution method was developed for the determination of streptococcus sanguis bacteriocins of P. g minimum bacteriostatic concentration （MIC）. We choose different concentrations of streptococcus sanguis bacteriocins affect P. g, respectively at 0, 4, 8, 12, 16, 20, 24 and 48 h to measure the determination of groups of bacteria suspension at 590 nm wavelength optical density （OD） value, and drew the growth eurve. We used Fluo--3/AM load P. g intraeellular calcium ions, and laser eonfocal microscope to observe Ca2＋ flu- orescence intensity changes. Results： Streptococcus sanguis bacteriocins has a significant inhibitory effect of P. g,the MIC is 0. 0625 g/L. After the Streptococcus sanguis bacteriocins works, P. g intracellular calcium ion concentration decreased significantly. The growth curve of P. g has a significant change occurred in 8 - 16 h, logarithmic phase is delayed for eight hours. The intracellular calcium ion concentration of . P. g significantly reduced, statistically significant difference compared with the control group（P〈0.05）. Conclusion： Streptococcus sanguis bacteriocins for P. g has obvious inhibition. Within the scope of this study, with the increase of streptococcus sanguis bacteriocins its inhibition enhanced gradually. Streptococcus sanguis bacteriocins can cause P. g cell membrane permeability enhaneed calcium bacterium.
出处
《口腔医学研究》
CAS
CSCD
2014年第2期108-111,共4页
Journal of Oral Science Research
基金
黑龙江省自然科学基金资助项目(D2007-03)
关键词
血链球菌细菌素
牙龈卟啉单胞菌
细胞内钙离子浓度
生长曲线
Streptococcus sanguis bacteriocins cium concentration Porphyromonas gingivalis Growth curve Intracellular free cal
