摘要
目的研究穿心莲内酯(ANDRO)对阿尔茨海默病(AD)大鼠血小板活化因子(PAF)及β淀粉样蛋白的影响。方法将30只SD大鼠随机分为治疗组(10只)、模型组(10只)、假手术组(10只)。采用侧脑室注射链脲霉素(STZ)制做AD大鼠模型。治疗组大鼠给予以ANDRO(200mg/kg)治疗。用Morris’s水迷宫评估大鼠认知功能,采用酶免法测定大鼠血浆PAF含量,免疫组化法检测大鼠海马Aβ表达。结果治疗组大鼠水迷宫潜伏期短于模型组但长于假手术组(P<0.05)。模型组、治疗组、假手术组穿过平台次数分别为(1.90+1.10)、(5.60+1.65),(3.70+1.56),(F=16.10,P<0.05)。模型组、治疗组、假手术组大鼠血浆PAF含量分别为(210.30±15.18)pg/ml、(182.90±12.08)pg/ml、(167.50+10.31)pg/ml,两两比较差异均有统计学意义(F=29.21,P<0.05)。模型组、治疗组、假手术组海马Aβ灰度值分别为(131.71±8.83)、(144.93±9.71)、(164.21±9.05),两两比较差异均有统计学意义(F=31.52,P<0.05)。结论 ANDRO可能通过下调AD大鼠血浆PAF及海马β淀粉样蛋白的表达进而改善认知功能。
Objective To investigate the effects of andrographollde （ANDRO） on platelet - activating factor （PAF） and amyloid -beta protein （Aβ） in rats with Alzheimerk disease（AD）. Methods 30 Sprague -Dawley（SD） rats were randomly assigned to treatment group （n = 10）, model group（n = 10） and sham - operation group（n = 10）. AD rats model was established by injecting streptozotocin （STZ） intracerebroventricularly. ANDRO （ 200 mg/kg） was given to treatment group. The rats＇cognitive ability was assessed by Morris＇s water maze test. ELISA was used for detecting the level of PAF, and immunohistocbemical staining methods for Aβ expression in hippocarnpus. Results Morrisk water maze test revealed that the mean duration of escape latency in treatment .group was shorter than that in model group, longer than that in sham -operation group（ P 〈 0.05 ）. The frequency of rats crossing the removed platform was （1.90 ± 1. 10）in model group, （3. 70 ± 1.56 ） in treatment group and （ 5. 60 ± 1.65 ） in sham - operation group, showing significant difference between the groups （F=16. 10, P〈0.05）.The concentration of PAF was （210.30 ± 15.18） μg/nd in model group, （182.90 ± 12. 08） μg/ml in treatment group and （ 167.50 ± 10.31 ） μg/ml in sham - operation group, showing significant differences between the groups （ F = 29. 21, P 〈 0. 05）. The grey value of Aβ in rat lfippecampus was （ 131.71 ± 8.83 ） in model group, （144.93 ± 9.71 ） in treatment group and （164.21 ± 9.05 ） in sham -operation group, showing significant differences between the groups （ F = 31.52, P 〈 0.05）. Conclusions ANDRO may improve the cognitive ability of rats with AD by lowering plasma concentration of PAF and expression of Aβ in the hippocampus.
出处
《社区医学杂志》
2014年第4期16-19,共4页
Journal Of Community Medicine
